Dlk1 in normal and abnormal hematopoiesis

S. Sakajiri; J. O'Kelly; D. Yin; C. W. Miller; W. K. Hofmann; K. Oshimi; L. Y. Shih; K. H. Kim; H. S. Sul; C. H. Jensen; B. Teisner; Norihiko Kawamata; H. P. Koeffler

doi:10.1038/sj.leu.2403832

Dlk1 in normal and abnormal hematopoiesis

S. Sakajiri, J. O'Kelly, D. Yin, C. W. Miller, W. K. Hofmann, K. Oshimi, L. Y. Shih, K. H. Kim, H. S. Sul, C. H. Jensen, B. Teisner, Norihiko Kawamata^*, H. P. Koeffler

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

81 Scopus citations

Abstract

Dlk1 (Pref-1) is a transmembrane and secreted protein, which is a member of the epidermal growth factor-like family, homologous to Notch/Delta/Serrate. We have found by real-time RT-PCR that Dlk1 mRNA levels were high in CD34⁺ cells in 10 of 12 MDS samples compared with CD34⁺ cells from 11 normals. Also, Dlk1 mRNA was elevated in mononuclear, low density bone marrow cells from 11/38 MDS patients, 5/11 AML M6 and 2/4 AML M7 samples. Furthermore, 5/6 erythroleukemia and 2/2 megakaryocytic leukemia cell lines highly expressed Dlk1 mRNA. Levels of Dlk1 mRNA markedly increased during megakaryocytic differentiation of both CMK megakaryoblasts as well as normal CD34⁺ hematopoietic stem cells. High serum levels of Dlk1 occurred in RA (4/10) and essential thrombocythemia (2/10) patients. Functional studies showed that forced expression of Dlk1 enhanced proliferation of K562 cells growing in 1% fetal bovine serum. Analysis of hematopoiesis of Dlk1 knockout mice suggested that Dlk1 contributed to granulocyte, megakaryocyte and B-cell clonogenic growth and was needed for generation of splenic B-cells. In summary, Dlk1 is overexpressed in selected samples of MDS (especially RA and RAEB) and AML (particularly M6, M7), and it appears to be associated with normal development of megakaryocytes and B cells.

Original language	English
Pages (from-to)	1404-1410
Number of pages	7
Journal	Leukemia
Volume	19
Issue number	8
DOIs	https://doi.org/10.1038/sj.leu.2403832
State	Published - 2005

Keywords

B-cell development
Erythroleukemia
MDS
Megakaryocyte
Real-time PCR

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@article{9868d9fc222d47bb9e266543d1f318b0,

title = "Dlk1 in normal and abnormal hematopoiesis",

abstract = "Dlk1 (Pref-1) is a transmembrane and secreted protein, which is a member of the epidermal growth factor-like family, homologous to Notch/Delta/Serrate. We have found by real-time RT-PCR that Dlk1 mRNA levels were high in CD34+ cells in 10 of 12 MDS samples compared with CD34+ cells from 11 normals. Also, Dlk1 mRNA was elevated in mononuclear, low density bone marrow cells from 11/38 MDS patients, 5/11 AML M6 and 2/4 AML M7 samples. Furthermore, 5/6 erythroleukemia and 2/2 megakaryocytic leukemia cell lines highly expressed Dlk1 mRNA. Levels of Dlk1 mRNA markedly increased during megakaryocytic differentiation of both CMK megakaryoblasts as well as normal CD34+ hematopoietic stem cells. High serum levels of Dlk1 occurred in RA (4/10) and essential thrombocythemia (2/10) patients. Functional studies showed that forced expression of Dlk1 enhanced proliferation of K562 cells growing in 1\% fetal bovine serum. Analysis of hematopoiesis of Dlk1 knockout mice suggested that Dlk1 contributed to granulocyte, megakaryocyte and B-cell clonogenic growth and was needed for generation of splenic B-cells. In summary, Dlk1 is overexpressed in selected samples of MDS (especially RA and RAEB) and AML (particularly M6, M7), and it appears to be associated with normal development of megakaryocytes and B cells.",

keywords = "B-cell development, Erythroleukemia, MDS, Megakaryocyte, Real-time PCR",

author = "S. Sakajiri and J. O'Kelly and D. Yin and Miller, \{C. W.\} and Hofmann, \{W. K.\} and K. Oshimi and Shih, \{L. Y.\} and Kim, \{K. H.\} and Sul, \{H. S.\} and Jensen, \{C. H.\} and B. Teisner and Norihiko Kawamata and Koeffler, \{H. P.\}",

year = "2005",

doi = "10.1038/sj.leu.2403832",

language = "英语",

volume = "19",

pages = "1404--1410",

journal = "Leukemia",

issn = "0887-6924",

publisher = "Springer Nature",

number = "8",

}

TY - JOUR

T1 - Dlk1 in normal and abnormal hematopoiesis

AU - Sakajiri, S.

AU - O'Kelly, J.

AU - Yin, D.

AU - Miller, C. W.

AU - Hofmann, W. K.

AU - Oshimi, K.

AU - Shih, L. Y.

AU - Kim, K. H.

AU - Sul, H. S.

AU - Jensen, C. H.

AU - Teisner, B.

AU - Kawamata, Norihiko

AU - Koeffler, H. P.

PY - 2005

Y1 - 2005

N2 - Dlk1 (Pref-1) is a transmembrane and secreted protein, which is a member of the epidermal growth factor-like family, homologous to Notch/Delta/Serrate. We have found by real-time RT-PCR that Dlk1 mRNA levels were high in CD34+ cells in 10 of 12 MDS samples compared with CD34+ cells from 11 normals. Also, Dlk1 mRNA was elevated in mononuclear, low density bone marrow cells from 11/38 MDS patients, 5/11 AML M6 and 2/4 AML M7 samples. Furthermore, 5/6 erythroleukemia and 2/2 megakaryocytic leukemia cell lines highly expressed Dlk1 mRNA. Levels of Dlk1 mRNA markedly increased during megakaryocytic differentiation of both CMK megakaryoblasts as well as normal CD34+ hematopoietic stem cells. High serum levels of Dlk1 occurred in RA (4/10) and essential thrombocythemia (2/10) patients. Functional studies showed that forced expression of Dlk1 enhanced proliferation of K562 cells growing in 1% fetal bovine serum. Analysis of hematopoiesis of Dlk1 knockout mice suggested that Dlk1 contributed to granulocyte, megakaryocyte and B-cell clonogenic growth and was needed for generation of splenic B-cells. In summary, Dlk1 is overexpressed in selected samples of MDS (especially RA and RAEB) and AML (particularly M6, M7), and it appears to be associated with normal development of megakaryocytes and B cells.

AB - Dlk1 (Pref-1) is a transmembrane and secreted protein, which is a member of the epidermal growth factor-like family, homologous to Notch/Delta/Serrate. We have found by real-time RT-PCR that Dlk1 mRNA levels were high in CD34+ cells in 10 of 12 MDS samples compared with CD34+ cells from 11 normals. Also, Dlk1 mRNA was elevated in mononuclear, low density bone marrow cells from 11/38 MDS patients, 5/11 AML M6 and 2/4 AML M7 samples. Furthermore, 5/6 erythroleukemia and 2/2 megakaryocytic leukemia cell lines highly expressed Dlk1 mRNA. Levels of Dlk1 mRNA markedly increased during megakaryocytic differentiation of both CMK megakaryoblasts as well as normal CD34+ hematopoietic stem cells. High serum levels of Dlk1 occurred in RA (4/10) and essential thrombocythemia (2/10) patients. Functional studies showed that forced expression of Dlk1 enhanced proliferation of K562 cells growing in 1% fetal bovine serum. Analysis of hematopoiesis of Dlk1 knockout mice suggested that Dlk1 contributed to granulocyte, megakaryocyte and B-cell clonogenic growth and was needed for generation of splenic B-cells. In summary, Dlk1 is overexpressed in selected samples of MDS (especially RA and RAEB) and AML (particularly M6, M7), and it appears to be associated with normal development of megakaryocytes and B cells.

KW - B-cell development

KW - Erythroleukemia

KW - MDS

KW - Megakaryocyte

KW - Real-time PCR

UR - https://www.scopus.com/pages/publications/23744486278

U2 - 10.1038/sj.leu.2403832

DO - 10.1038/sj.leu.2403832

M3 - 文章

C2 - 15959531

AN - SCOPUS:23744486278

SN - 0887-6924

VL - 19

SP - 1404

EP - 1410

JO - Leukemia

JF - Leukemia

IS - 8

ER -

Dlk1 in normal and abnormal hematopoiesis

Abstract

Keywords

UN SDGs

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