Dual effect of the antianginal drug fendiline on bladder female transitional carcinoma cells: Mobilization of intracellular Ca2+ and induction of cell death

Chung Ren Jan, Chia Cheng Yu, Jong Khing Huang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

The effect of fendiline, an antianginal drug, on cytosolic free Ca2+ levels ([Ca2+]i) in populations of bladder female transitional carcinoma (BFTC) cells was explored using fura-2 as a Ca 2+ indicator. Fendiline at concentrations between 3 and 200 μmol/l increased [Ca2+]i in a concentration-dependent manner and the signal saturated at 100 μmol/l. The [Ca2+]i signal was biphasic, with an initial rise and a slow decay. Ca2+ removal inhibited the Ca2+ signal by about half in peak amplitude. Adding 3 mmol/l Ca2+ increased [Ca2+]i in cells pretreated with 100 μmol/l fendiline in Ca2+-free medium, suggesting that fendiline induced Ca2+ influx via capacitative Ca2+ entry. In Ca2+-free medium, pretreatment with 1 μmol/l thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) to deplete the endoplasmic reticulum Ca2+ store inhibited most of the 100 μmol/l fendiline-induced internal Ca2+ release; and conversely, pretreatment with 100 μmol/l fendiline partly inhibited 1 μmol/l thapsigargin-induced Ca2+ release. This indicates that the major internal Ca2+ store of fendiline-induced [Ca2+]i increases is located in the endoplasmic reticulum. The Ca2+ release induced by 100 μmol/l fendiline may be partly mediated by inositol 1,4,5-trisphosphate, because the [Ca2+]i increase was inhibited by 50% by inhibiting phospholipase C with 2 μmol/l U73122. Fendiline (100 μmol/l) decreased cell viability by 12-44% after being added to cells for 2-30 min. Together, the findings indicate that in BFTC cells, fendiline exerts a dual effect: mobilization of intracellular Ca2+ and induction of cell death.

Original languageEnglish
Pages (from-to)218-223
Number of pages6
JournalPharmacology
Volume62
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • BFTC cells
  • Bladder cell carcinoma
  • Ca signaling
  • Fendiline
  • Fura-2

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