Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system

Rou Xuan Huang; Ying Fu; Wang Liu; Yu Ting Ma; Bao Yu Hsieh; Shu Ching Chen; Mingjian Sun; Pai Chi Li

doi:10.1117/12.2289975

Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system

Rou Xuan Huang
, Ying Fu
, Wang Liu
, Yu Ting Ma
, Bao Yu Hsieh
, Shu Ching Chen
, Mingjian Sun
, Pai Chi Li^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm³. To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs.

Original language	English
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2018
Editors	Lihong V. Wang, Alexander A. Oraevsky
Publisher	SPIE
ISBN (Electronic)	9781510614734
DOIs	https://doi.org/10.1117/12.2289975
State	Published - 2018
Externally published	Yes
Event	Photons Plus Ultrasound: Imaging and Sensing 2018 - San Francisco, United States Duration: 28 01 2018 → 01 02 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10494
ISSN (Print)	1605-7422

Conference

Conference	Photons Plus Ultrasound: Imaging and Sensing 2018
Country/Territory	United States
City	San Francisco
Period	28/01/18 → 01/02/18

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

3D Cell Culture
Compressed Sensing
Matrix Completion
Photoacoustic Microscopy
Sparse Sampling

Access to Document

10.1117/12.2289975

Cite this

Huang, R. X., Fu, Y., Liu, W., Ma, Y. T., Hsieh, B. Y., Chen, S. C., Sun, M., & Li, P. C. (2018). Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system. In L. V. Wang, & A. A. Oraevsky (Eds.), Photons Plus Ultrasound: Imaging and Sensing 2018 Article 1049462 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10494). SPIE. https://doi.org/10.1117/12.2289975

@inproceedings{b3aea090e3204422a65ade31228d5dc8,

title = "Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system",

abstract = "Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm3. To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40\% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs.",

keywords = "3D Cell Culture, Compressed Sensing, Matrix Completion, Photoacoustic Microscopy, Sparse Sampling",

author = "Huang, \{Rou Xuan\} and Ying Fu and Wang Liu and Ma, \{Yu Ting\} and Hsieh, \{Bao Yu\} and Chen, \{Shu Ching\} and Mingjian Sun and Li, \{Pai Chi\}",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Photons Plus Ultrasound: Imaging and Sensing 2018 ; Conference date: 28-01-2018 Through 01-02-2018",

year = "2018",

doi = "10.1117/12.2289975",

language = "英语",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Wang, \{Lihong V.\} and Oraevsky, \{Alexander A.\}",

booktitle = "Photons Plus Ultrasound",

address = "美国",

}

Huang, RX, Fu, Y, Liu, W, Ma, YT, Hsieh, BY, Chen, SC, Sun, M & Li, PC 2018, Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system. in LV Wang & AA Oraevsky (eds), Photons Plus Ultrasound: Imaging and Sensing 2018., 1049462, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10494, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2018, San Francisco, United States, 28/01/18. https://doi.org/10.1117/12.2289975

Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system. / Huang, Rou Xuan; Fu, Ying; Liu, Wang et al.
Photons Plus Ultrasound: Imaging and Sensing 2018. ed. / Lihong V. Wang; Alexander A. Oraevsky. SPIE, 2018. 1049462 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10494).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system

AU - Huang, Rou Xuan

AU - Fu, Ying

AU - Liu, Wang

AU - Ma, Yu Ting

AU - Hsieh, Bao Yu

AU - Chen, Shu Ching

AU - Sun, Mingjian

AU - Li, Pai Chi

PY - 2018

Y1 - 2018

N2 - Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm3. To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs.

AB - Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm3. To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs.

KW - 3D Cell Culture

KW - Compressed Sensing

KW - Matrix Completion

KW - Photoacoustic Microscopy

KW - Sparse Sampling

UR - https://www.scopus.com/pages/publications/85047366525

U2 - 10.1117/12.2289975

DO - 10.1117/12.2289975

M3 - 会议稿件

AN - SCOPUS:85047366525

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Photons Plus Ultrasound

A2 - Wang, Lihong V.

A2 - Oraevsky, Alexander A.

PB - SPIE

T2 - Photons Plus Ultrasound: Imaging and Sensing 2018

Y2 - 28 January 2018 through 1 February 2018

ER -

Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Keywords

Access to Document

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