Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

Albert M. Wu; Yu Ping Gong; Chia Chen Li; Nechama Gilboa-Garber

doi:10.1016/j.febslet.2010.04.026

Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

Albert M. Wu^*
, Yu Ping Gong
, Chia Chen Li
, Nechama Gilboa-Garber

^*Corresponding author for this work

Department of Molecular Biology

Research output: Contribution to journal › Journal Article › peer-review

12 Scopus citations

Abstract

The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

Original language	English
Pages (from-to)	2371-2375
Number of pages	5
Journal	FEBS Letters
Volume	584
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2010.04.026
State	Published - 06 2010

Keywords

Bacterial lectin
Carbohydrate-recognition
Glycoprotein
N-glycan
Pseudomonas aeruginosa
Yeast mannan

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title = "Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)",

abstract = "The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.",

keywords = "Bacterial lectin, Carbohydrate-recognition, Glycoprotein, N-glycan, Pseudomonas aeruginosa, Yeast mannan",

author = "Wu, \{Albert M.\} and Gong, \{Yu Ping\} and Li, \{Chia Chen\} and Nechama Gilboa-Garber",

year = "2010",

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doi = "10.1016/j.febslet.2010.04.026",

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journal = "FEBS Letters",

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}

TY - JOUR

T1 - Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

AU - Wu, Albert M.

AU - Gong, Yu Ping

AU - Li, Chia Chen

AU - Gilboa-Garber, Nechama

PY - 2010/6

Y1 - 2010/6

N2 - The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

AB - The study of Pseudomonas aeruginosa-II lectin (PA-IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA-IIL carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak -OH conformation as seen in yeast mannan is also an important PA-IIL recognition factor. This finding provides a peculiar concept of the duality of PA-IIL recognition system for lFucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.

KW - Bacterial lectin

KW - Carbohydrate-recognition

KW - Glycoprotein

KW - N-glycan

KW - Pseudomonas aeruginosa

KW - Yeast mannan

UR - https://www.scopus.com/pages/publications/77952916002

U2 - 10.1016/j.febslet.2010.04.026

DO - 10.1016/j.febslet.2010.04.026

M3 - 文章

C2 - 20398656

AN - SCOPUS:77952916002

SN - 0014-5793

VL - 584

SP - 2371

EP - 2375

JO - FEBS Letters

JF - FEBS Letters

IS - 11

ER -

Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL)

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Keywords

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