Dynamic embryo culture on a digital microfluidic chip

Chun Chao Li, Yao Wen Hsu, Hong Yuan Huang, Shih Kang Fan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

Embryo culture on a digital microfluidic Chip is presented. The digital microfluidic chip consists of two parallel glass plates with conductive., dielectric., and hydrophobic coatings. ICR mouse embryos are cultivated in the culture media (KSOM AA) droplets placed in a petri dish for static studies or between the parallel glass plates for dynamic studies. Embryo culture is successful with the conductive ITO., dielectric SU-8 2002., hydrophobic Teflon layers., and surfactant Pluronic F127 used in the digital microfluidic devices. Moreover., the blastocyst rate of the embryos cultivated in encapsulated droplets containing culture media core droplets and appropriate culture oil shells is comparable to that of the embryos cultivated in traditional petri dishes. Electrowetting-on-dielectric (EWOD) effectively actuates the investigated encapsulated droplets that will be used for dynamic embryo culture.

Original languageEnglish
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages74-77
Number of pages4
DOIs
StatePublished - 2012
Externally publishedYes
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: 04 11 201207 11 2012

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

Conference6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
Country/TerritoryThailand
CityBangkok
Period04/11/1207/11/12

Keywords

  • Digital microfluidics
  • Electrowetting-on-dielectric (EWOD)
  • Embryo culture

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