TY - JOUR
T1 - Dynorphin uses a non-opioid mechanism to potentiate N-methyl-D- aspartate currents in single rat periaqueductal gray neurons
AU - Lai, Shung Lon
AU - Gu, Yanping
AU - Huang, Li Yen Mae
PY - 1998/5/15
Y1 - 1998/5/15
N2 - The interaction of the endogenous K-opioid, dynorphin, with N-methyl-D- aspartate (NMDA) receptors was studied in single periaqueductal gray (PAG) cells using the whole cell patch recording technique. We have found that dynorphin A (1-17) rapidly and reversibly potentiates NMDA-activated currents in a subpopulation of PAG cells. The potentiation cannot be blocked by the non-specific opioid antagonist, naloxone, nor can it be reversed by the specific κ-opioid antagonist, nor-BNI. In addition, the non-opioid fragment of dynorphin, dynorphin A (2-17), is effective in potentiating NMDA currents, while the specific κ-opioid, U50,488, cannot mimic the action of dynorphin A (1-17). The non-opioid dynorphin action and the rapid onset and recovery of the potentiation are consistent with the idea that dynorphin interacts directly with NMDA receptors in PAG cells.
AB - The interaction of the endogenous K-opioid, dynorphin, with N-methyl-D- aspartate (NMDA) receptors was studied in single periaqueductal gray (PAG) cells using the whole cell patch recording technique. We have found that dynorphin A (1-17) rapidly and reversibly potentiates NMDA-activated currents in a subpopulation of PAG cells. The potentiation cannot be blocked by the non-specific opioid antagonist, naloxone, nor can it be reversed by the specific κ-opioid antagonist, nor-BNI. In addition, the non-opioid fragment of dynorphin, dynorphin A (2-17), is effective in potentiating NMDA currents, while the specific κ-opioid, U50,488, cannot mimic the action of dynorphin A (1-17). The non-opioid dynorphin action and the rapid onset and recovery of the potentiation are consistent with the idea that dynorphin interacts directly with NMDA receptors in PAG cells.
KW - Dynorphin
KW - Excitatory opioid action
KW - N-Methyl-D-aspartate receptor
KW - Non-opioid
KW - Periaqueductal gray
KW - κ-Opioid
UR - http://www.scopus.com/inward/record.url?scp=0032524331&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(98)00293-6
DO - 10.1016/S0304-3940(98)00293-6
M3 - 文章
C2 - 9655606
AN - SCOPUS:0032524331
SN - 0304-3940
VL - 247
SP - 115
EP - 118
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2-3
ER -