Dysfunction of methionine sulfoxide reductases to repair damaged proteins by nickel nanoparticles

Po Hao Feng*, Ya Li Huang, Kai Jen Chuang, Kuan Yuan Chen, Kang Yun Lee, Shu Chuan Ho, Mauo Ying Bien, You Lan Yang, Hsiao Chi Chuang

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

11 Scopus citations

Abstract

Background: Protein oxidation is considered to be one of the main causes of cell death, and methionine is one of the primary targets of reactive oxygen species (ROS). However, the mechanisms by which nickel nanoparticles (NiNPs) cause oxidative damage to proteins remain unclear. Objectives: The objective of this study is to investigate the effects of NiNPs on the methionine sulfoxide reductases (MSR) protein repairing system. Methods: Two physically similar nickel-based nanoparticles, NiNPs and carbon-coated NiNP (C-NiNPs; control particles), were exposed to human epithelial A549 cells. Cell viability, benzo(a)pyrene diolepoxide (BPDE) protein adducts, methionine oxidation, MSRA and B3, microtubule-associated protein 1A/1B-light chain 3 (LC3) and extracellular signal-regulated kinase (ERK) phosphorylation were investigated. Results: Exposure to NiNPs led to a dose-dependent reduction in cell viability and increased BPDE protein adduct production and methionine oxidation. The methionine repairing enzymatic MSRA and MSRB3 production were suppressed in response to NiNP exposure, suggesting the oxidation of methionine to MetO by NiNP was not reversed back to methionine. Additionally, LC3, an autophagy marker, was down-regulated by NiNPs. Both NiNP and C-NiNP caused ERK phosphorylation. LC3 was positively correlated with MSRA (r = 0.929, p < 0.05) and MSRB3 (r = 0.893, p < 0.05). Conclusions: MSR was made aberrant by NiNP, which could lead to the dysfunction of autophagy and ERK phosphorylation. The toxicological consequences may be dependent on the chemical characteristics of the nanoparticles.

Original languageEnglish
Pages (from-to)82-89
Number of pages8
JournalChemico-Biological Interactions
Volume236
DOIs
StatePublished - 16 05 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ireland Ltd. All rights reserved.

Keywords

  • Autophagy
  • Benzo(a)pyrene diolepoxide protein adduct
  • Extracellular signal-regulated kinase
  • Methionine oxidation
  • Methionine sulfoxide reductases
  • Nickel nanoparticle

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