E5 proteins of human papillomavirus types 11 and 16 transactivate the c- fos promoter through the NF1 binding element

Show Li Chen*, Ying Kuang Lin, Long Yuan Li, Yeou Ping Tsao, Hsiang Yun Lo, Won Bo Wang, Tzung Chieh Tsai

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

24 Scopus citations

Abstract

Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or HPV-16. The mutant studies show that NF1 binding sequences within the c-fos promoter were crucial for the induction of the c-fos gene by E5, and the gel shift assay study suggested that E5 of both HPV-11 and HPV-16 is associated, perhaps indirectly, with this NF1 element in the transactivation of the human c-fos promoter. Using an inducible system, we demonstrate that increased induction of the HPV-11 E5 gene in cells led to increased transactivation of the NF1 element. In addition, the transactivating activity of a series of HPV-11 E5 mutants on the NF1 element had a strong correlation with their respective transforming activities.

Original languageEnglish
Pages (from-to)8558-8563
Number of pages6
JournalJournal of Virology
Volume70
Issue number12
DOIs
StatePublished - 12 1996
Externally publishedYes

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