Effect of A-scan rate and interscan interval on optical coherence angiography

Ting Hao Chen, Yi Chun Wu, Ting Yen Tsai, Chuan Bor Chueh, Bo Huei Huang, Yin Peng Huang, Meng Tsan Tsai, Yoshiaki Yasuno, Hsiang Chieh Lee

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

Optical coherence tomography angiography (OCTA) can provide rapid, volumetric, and noninvasive imaging of tissue microvasculature without the requirement of exogenous contrast agents. To investigate how A-scan rate and interscan time affected the contrast and dynamic range of OCTA, we developed a 1.06-µm swept-source OCT system enabling 100-kHz or 200-kHz OCT using two light sources. After system settings were carefully adjusted, almost the same detection sensitivity was achieved between the 100-kHz and 200-kHz modalities. OCTA of ear skin was performed on five mice. We used the variable interscan time analysis algorithm (VISTA) and the designated scanning protocol with OCTA images reconstructed through the correlation mapping method. With a relatively long interscan time (e.g., 12.5 ms vs. 6.25 ms for 200-kHz OCT), OCTA can identify more intricate microvascular networks. OCTA image sets with the same interscan time (e.g., 12.5 ms) were compared. OCTA images acquired with a 100-kHz A-scan rate showed finer microvasculature than did other imaging modalities. We performed quantitative analysis on the contrast from OCTA images reconstructed with different A-scan rates and interscan time intervals in terms of vessel area, total vessel length, and junction density.

Original languageEnglish
Pages (from-to)722-736
Number of pages15
JournalBiomedical Optics Express
Volume12
Issue number2
DOIs
StatePublished - 2021

Bibliographical note

Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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