Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells

Shih Min Hsia; Yi Wen Tseng; Shyi Wu Wang; Yueh Hsiung Kuo; Din Wen Huang; Paulus S. Wang; Wenchang Chiang

doi:10.1002/ptr.2706

Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells

Shih Min Hsia, Yi Wen Tseng, Shyi Wu Wang, Yueh Hsiung Kuo, Din Wen Huang, Paulus S. Wang, Wenchang Chiang^*

^*Corresponding author for this work

Department of Physiology

Research output: Contribution to journal › Journal Article › peer-review

20 Scopus citations

Abstract

Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

Original language	English
Pages (from-to)	687-695
Number of pages	9
Journal	Phytotherapy Research
Volume	23
Issue number	5
DOIs	https://doi.org/10.1002/ptr.2706
State	Published - 05 2009

Keywords

Adlay hulls
Leydig cell
Testosterone

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10.1002/ptr.2706

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title = "Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells",

abstract = "Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.",

keywords = "Adlay hulls, Leydig cell, Testosterone",

author = "Hsia, {Shih Min} and Tseng, {Yi Wen} and Wang, {Shyi Wu} and Kuo, {Yueh Hsiung} and Huang, {Din Wen} and Wang, {Paulus S.} and Wenchang Chiang",

year = "2009",

month = may,

doi = "10.1002/ptr.2706",

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AU - Hsia, Shih Min

AU - Tseng, Yi Wen

AU - Wang, Shyi Wu

AU - Kuo, Yueh Hsiung

AU - Huang, Din Wen

AU - Wang, Paulus S.

AU - Chiang, Wenchang

PY - 2009/5

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N2 - Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

AB - Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3′,5′-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17β-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

KW - Adlay hulls

KW - Leydig cell

KW - Testosterone

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DO - 10.1002/ptr.2706

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SN - 0951-418X

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Effect of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) hull extracts on testosterone release from rat leydig cells

Abstract

Keywords

UN SDGs

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