Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application

Chu Hua Chiu; Han Chin Shih; Shyh Chuan Jwo; Ming Fa Hsieh

doi:10.1007/978-3-642-03900-3_85

Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application

Chu Hua Chiu
, Han Chin Shih
, Shyh Chuan Jwo
, Ming Fa Hsieh^*

^*Corresponding author for this work

Chung Yuan Christian University
Chang Gung Memorial Hospital

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

The present study aims to fabricate a tubular scaffold for the application of tissue engineering of small intestine. Gelatin solutions with concentrations of 10, 15, and 20 wt% were cross-linked using glutaraldehyde (GA) and 1-ethyl- 3-3-dimethylaminopropyl carbodiimide hydrochloride (EDC) in a mold to give hollow tubes. The extent of crosslinking reached 80 % when EDC was used, whereas it reached only 30% using GA. When observed under a scanning electron microscope (SEM), the outer surface of EDC-crosslinked gelatin tube was found to be smoother than that of the GA groups. However, the inner surfaces of both EDC and GA groups were smooth. The swelling ratio increased to 600 % in GA groups, and 300 % in EDC groups. A significant difference was shown in the Young's modulus of GA-and EDC-crosslinked tubes composed of 20 wt% gelatin. The present work provides a method of fabrication of crosslinked gelatin hollow tubes. For further study, we are comparing the static and dynamic culture of smooth muscle cells in the gelatin hollow tubes.

Original language	English
Title of host publication	World Congress on Medical Physics and Biomedical Engineering
Subtitle of host publication	Biomaterials, Cellular and Tissue Engineering, Artificial Organs
Publisher	Springer Verlag
Pages	293-296
Number of pages	4
Edition	10
ISBN (Print)	9783642038990
DOIs	https://doi.org/10.1007/978-3-642-03900-3_85
State	Published - 2009
Externally published	Yes
Event	World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs - Munich, Germany Duration: 07 09 2009 → 12 09 2009

Publication series

Name	IFMBE Proceedings
Number	10
Volume	25
ISSN (Print)	1680-0737

Conference

Conference	World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs
Country/Territory	Germany
City	Munich
Period	07/09/09 → 12/09/09

Keywords

Crosslinking
Gelatin
Hollow tube
Tissue engineering

Access to Document

10.1007/978-3-642-03900-3_85

Cite this

Chiu, C. H., Shih, H. C., Jwo, S. C., & Hsieh, M. F. (2009). Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application. In World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs (10 ed., pp. 293-296). (IFMBE Proceedings; Vol. 25, No. 10). Springer Verlag. https://doi.org/10.1007/978-3-642-03900-3_85

@inproceedings{e90a2448444a44e7bfcdf813552dce5b,

title = "Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application",

abstract = "The present study aims to fabricate a tubular scaffold for the application of tissue engineering of small intestine. Gelatin solutions with concentrations of 10, 15, and 20 wt\% were cross-linked using glutaraldehyde (GA) and 1-ethyl- 3-3-dimethylaminopropyl carbodiimide hydrochloride (EDC) in a mold to give hollow tubes. The extent of crosslinking reached 80 \% when EDC was used, whereas it reached only 30\% using GA. When observed under a scanning electron microscope (SEM), the outer surface of EDC-crosslinked gelatin tube was found to be smoother than that of the GA groups. However, the inner surfaces of both EDC and GA groups were smooth. The swelling ratio increased to 600 \% in GA groups, and 300 \% in EDC groups. A significant difference was shown in the Young's modulus of GA-and EDC-crosslinked tubes composed of 20 wt\% gelatin. The present work provides a method of fabrication of crosslinked gelatin hollow tubes. For further study, we are comparing the static and dynamic culture of smooth muscle cells in the gelatin hollow tubes.",

keywords = "Crosslinking, Gelatin, Hollow tube, Tissue engineering",

author = "Chiu, \{Chu Hua\} and Shih, \{Han Chin\} and Jwo, \{Shyh Chuan\} and Hsieh, \{Ming Fa\}",

year = "2009",

doi = "10.1007/978-3-642-03900-3\_85",

language = "英语",

isbn = "9783642038990",

series = "IFMBE Proceedings",

publisher = "Springer Verlag",

number = "10",

pages = "293--296",

booktitle = "World Congress on Medical Physics and Biomedical Engineering",

address = "德国",

edition = "10",

note = "World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs ; Conference date: 07-09-2009 Through 12-09-2009",

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Chiu, CH, Shih, HC, Jwo, SC & Hsieh, MF 2009, Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application. in World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs. 10 edn, IFMBE Proceedings, no. 10, vol. 25, Springer Verlag, pp. 293-296, World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs, Munich, Germany, 07/09/09. https://doi.org/10.1007/978-3-642-03900-3_85

Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application. / Chiu, Chu Hua; Shih, Han Chin; Jwo, Shyh Chuan et al.
World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs. 10. ed. Springer Verlag, 2009. p. 293-296 (IFMBE Proceedings; Vol. 25, No. 10).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application

AU - Chiu, Chu Hua

AU - Shih, Han Chin

AU - Jwo, Shyh Chuan

AU - Hsieh, Ming Fa

PY - 2009

Y1 - 2009

N2 - The present study aims to fabricate a tubular scaffold for the application of tissue engineering of small intestine. Gelatin solutions with concentrations of 10, 15, and 20 wt% were cross-linked using glutaraldehyde (GA) and 1-ethyl- 3-3-dimethylaminopropyl carbodiimide hydrochloride (EDC) in a mold to give hollow tubes. The extent of crosslinking reached 80 % when EDC was used, whereas it reached only 30% using GA. When observed under a scanning electron microscope (SEM), the outer surface of EDC-crosslinked gelatin tube was found to be smoother than that of the GA groups. However, the inner surfaces of both EDC and GA groups were smooth. The swelling ratio increased to 600 % in GA groups, and 300 % in EDC groups. A significant difference was shown in the Young's modulus of GA-and EDC-crosslinked tubes composed of 20 wt% gelatin. The present work provides a method of fabrication of crosslinked gelatin hollow tubes. For further study, we are comparing the static and dynamic culture of smooth muscle cells in the gelatin hollow tubes.

AB - The present study aims to fabricate a tubular scaffold for the application of tissue engineering of small intestine. Gelatin solutions with concentrations of 10, 15, and 20 wt% were cross-linked using glutaraldehyde (GA) and 1-ethyl- 3-3-dimethylaminopropyl carbodiimide hydrochloride (EDC) in a mold to give hollow tubes. The extent of crosslinking reached 80 % when EDC was used, whereas it reached only 30% using GA. When observed under a scanning electron microscope (SEM), the outer surface of EDC-crosslinked gelatin tube was found to be smoother than that of the GA groups. However, the inner surfaces of both EDC and GA groups were smooth. The swelling ratio increased to 600 % in GA groups, and 300 % in EDC groups. A significant difference was shown in the Young's modulus of GA-and EDC-crosslinked tubes composed of 20 wt% gelatin. The present work provides a method of fabrication of crosslinked gelatin hollow tubes. For further study, we are comparing the static and dynamic culture of smooth muscle cells in the gelatin hollow tubes.

KW - Crosslinking

KW - Gelatin

KW - Hollow tube

KW - Tissue engineering

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T3 - IFMBE Proceedings

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EP - 296

BT - World Congress on Medical Physics and Biomedical Engineering

PB - Springer Verlag

T2 - World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs

Y2 - 7 September 2009 through 12 September 2009

ER -

Chiu CH, Shih HC, Jwo SC, Hsieh MF. Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application. In World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs. 10 ed. Springer Verlag. 2009. p. 293-296. (IFMBE Proceedings; 10). doi: 10.1007/978-3-642-03900-3_85

Effect of crosslinkers on physical properties of gelatin hollow tubes for tissue engineering application

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