Effect of divalent cations on DNA condensed on the surface of rigid cationic membrane

Ching Mao Wu; Chun Yu Chen; Szu Yin Lin; Hsin Lung Chen

doi:10.1016/j.reactfunctpolym.2010.09.009

Effect of divalent cations on DNA condensed on the surface of rigid cationic membrane

Ching Mao Wu, Chun Yu Chen, Szu Yin Lin, Hsin Lung Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

3 Scopus citations

Abstract

It has been indicated in previous literature reports that divalent cations cannot directly condense DNA in aqueous solution. However, several studies have shown that divalent metal ions can induce a further condensation of DNA originally adsorbed on the surfaces of fluid cationic membranes. In this article, we examine the effect of divalent metal ions (Ca²⁺ and Ni²⁺) on DNA condensed on the surfaces of a rigid cationic membrane formed by the mixture of cholesteryl 3-N-(dimethyl-aminoethyl) carbamate (DC-Chol) and 1,2-di(cis-9-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC). Our results demonstrate that the divalent cations exert an electrostatic screening effect between DNA, where the in-plane spacing between DNA (d_DNA) confined between the lipid bilayers gradually decreases with increasing cation concentration when the concentration exceeds a threshold value. The collapse transition, where d_DNA drops discontinuously at a critical cation concentration, observed previously for the fluid membrane is not identified here, indicating the critical role of membrane flexibility in the two-dimensional condensation of DNA.

Original language	English
Pages (from-to)	266-271
Number of pages	6
Journal	Reactive and Functional Polymers
Volume	71
Issue number	3
DOIs	https://doi.org/10.1016/j.reactfunctpolym.2010.09.009
State	Published - 03 2011
Externally published	Yes

Keywords

Cationic membrane
DNA condensation
Divalent cations
Electrostatic screening

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10.1016/j.reactfunctpolym.2010.09.009

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title = "Effect of divalent cations on DNA condensed on the surface of rigid cationic membrane",

abstract = "It has been indicated in previous literature reports that divalent cations cannot directly condense DNA in aqueous solution. However, several studies have shown that divalent metal ions can induce a further condensation of DNA originally adsorbed on the surfaces of fluid cationic membranes. In this article, we examine the effect of divalent metal ions (Ca2+ and Ni2+) on DNA condensed on the surfaces of a rigid cationic membrane formed by the mixture of cholesteryl 3-N-(dimethyl-aminoethyl) carbamate (DC-Chol) and 1,2-di(cis-9-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC). Our results demonstrate that the divalent cations exert an electrostatic screening effect between DNA, where the in-plane spacing between DNA (dDNA) confined between the lipid bilayers gradually decreases with increasing cation concentration when the concentration exceeds a threshold value. The collapse transition, where dDNA drops discontinuously at a critical cation concentration, observed previously for the fluid membrane is not identified here, indicating the critical role of membrane flexibility in the two-dimensional condensation of DNA.",

keywords = "Cationic membrane, DNA condensation, Divalent cations, Electrostatic screening",

author = "Wu, {Ching Mao} and Chen, {Chun Yu} and Lin, {Szu Yin} and Chen, {Hsin Lung}",

year = "2011",

month = mar,

doi = "10.1016/j.reactfunctpolym.2010.09.009",

language = "英语",

volume = "71",

pages = "266--271",

journal = "Reactive and Functional Polymers",

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publisher = "Elsevier B.V.",

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}

TY - JOUR

T1 - Effect of divalent cations on DNA condensed on the surface of rigid cationic membrane

AU - Wu, Ching Mao

AU - Chen, Chun Yu

AU - Lin, Szu Yin

AU - Chen, Hsin Lung

PY - 2011/3

Y1 - 2011/3

N2 - It has been indicated in previous literature reports that divalent cations cannot directly condense DNA in aqueous solution. However, several studies have shown that divalent metal ions can induce a further condensation of DNA originally adsorbed on the surfaces of fluid cationic membranes. In this article, we examine the effect of divalent metal ions (Ca2+ and Ni2+) on DNA condensed on the surfaces of a rigid cationic membrane formed by the mixture of cholesteryl 3-N-(dimethyl-aminoethyl) carbamate (DC-Chol) and 1,2-di(cis-9-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC). Our results demonstrate that the divalent cations exert an electrostatic screening effect between DNA, where the in-plane spacing between DNA (dDNA) confined between the lipid bilayers gradually decreases with increasing cation concentration when the concentration exceeds a threshold value. The collapse transition, where dDNA drops discontinuously at a critical cation concentration, observed previously for the fluid membrane is not identified here, indicating the critical role of membrane flexibility in the two-dimensional condensation of DNA.

AB - It has been indicated in previous literature reports that divalent cations cannot directly condense DNA in aqueous solution. However, several studies have shown that divalent metal ions can induce a further condensation of DNA originally adsorbed on the surfaces of fluid cationic membranes. In this article, we examine the effect of divalent metal ions (Ca2+ and Ni2+) on DNA condensed on the surfaces of a rigid cationic membrane formed by the mixture of cholesteryl 3-N-(dimethyl-aminoethyl) carbamate (DC-Chol) and 1,2-di(cis-9-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC). Our results demonstrate that the divalent cations exert an electrostatic screening effect between DNA, where the in-plane spacing between DNA (dDNA) confined between the lipid bilayers gradually decreases with increasing cation concentration when the concentration exceeds a threshold value. The collapse transition, where dDNA drops discontinuously at a critical cation concentration, observed previously for the fluid membrane is not identified here, indicating the critical role of membrane flexibility in the two-dimensional condensation of DNA.

KW - Cationic membrane

KW - DNA condensation

KW - Divalent cations

KW - Electrostatic screening

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U2 - 10.1016/j.reactfunctpolym.2010.09.009

DO - 10.1016/j.reactfunctpolym.2010.09.009

M3 - 文章

AN - SCOPUS:79551681953

SN - 1381-5148

VL - 71

SP - 266

EP - 271

JO - Reactive and Functional Polymers

JF - Reactive and Functional Polymers

IS - 3

ER -

Effect of divalent cations on DNA condensed on the surface of rigid cationic membrane

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Keywords

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