Effect of estrogen and 1α,25(OH)2- vitamin D3 on the activity and growth of human primary osteoblast-like cells in vitro

Fang Ping Chen*, Ning Lee, Kun Chuang Wang, Yung Kui Soong, Ko En Huang

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

24 Scopus citations

Abstract

Objective: To evaluate effects of 17β-E2 and 1α,25(OH)2-vitamin D3 on human osteoblast-like (hOB) cells. Design: Controlled, experimental study. Setting: University hospital. Patient(s): hOB cell cultures were prepared from the upper femur of postmenopausal patients undergoing bipolar endoprosthesis arthroplasty for a fractured femoral neck. Intervention(s): hOB cells were subcultured with either 17β-E2 or 1α,25(OH)2-vitamin D3, or both. Main Outcome Measure(s): Cell proliferation and activity of alkaline phosphatase, osteocalcin, and interleukin-6. Result(s): 17β-E2 significantly reduced interleukin-6 and osteocalcin to 34% and 60% of control value but induced alkaline phosphatase and cell proliferation to 183% and 150% of control value. 1α,25(OH)2-vitamin D3 significantly decreased cell proliferation to 88% of that of control group, but 1α,25(OH)2-vitamin D3 plus 17β-E2 showed no difference from the control group. Alkaline phosphatase and osteocalcin were significantly increased by 1α,25(OH)2-vitamin D3 alone or combined with 17β-E2, to 169% and 198% and to 144% and 144% of control value, respectively. 1α,25(OH)2-vitamin D3, with or without 17β-E2, decreased interleukin-6 levels to 27% and 38% of control group, respectively. Conclusion(s): 17β-E2 and 1α,25(OH)2-vitamin D3 have effects on osteoblasts. The prevention of osteoporosis by estrogen may be related not only to direct effects on osteoblastic activity and proliferation but also to indirect effects on osteoclasts by the decrease of interleukin-6 secretion.

Original languageEnglish
Pages (from-to)1038-1043
Number of pages6
JournalFertility and Sterility
Volume77
Issue number5
DOIs
StatePublished - 2002

Keywords

  • 17β-E
  • 25(OH)-vitamin D
  • Alkaline phosphatase
  • Human osteoblast-like cells
  • Interleukin-6
  • Osteocalcin

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