Effect of fluoxetine on intracellular Ca2+ levels in bladder female transitional carcinoma (BFTC) cells

Kwong Yui Tang, Tim Lu, Cheng Ho Chang, Yuk Keung Lo, Jin Shiung Cheng, Jue Long Wang, Hong Tai Chang, Chung Ren Jan*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

11 Scopus citations


The effect of the antidepressant fluoxetine on Ca2+ signaling in cultured cells was largely unknown. The effect of various concentrations of fluoxetine on [Ca2+]i in populations of bladder female transitional cancer (BFTC) cells was evaluated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration dependently (20-100 μM) with an EC50 value of 30 μM. The response was inhibited by 50-60% on extracellular Ca2+ removal. In CA2+-free medium, pretreatment with 1 μM thapsigargin (an inhibitor of the endoplasmic reticulum Ca2+ pump) abolished 50 μM fluoxetine-induced Ca2+ release; whereas pretreatment with fluoxetine did not alter the thapsigargin-induced Ca2+ response. Addition of 3 mM Ca2+ increased [Ca2+]i after pretreatment with 50 μM fluoxetine in Ca2+-free medium, suggestive of fluoxetine-induced capacitative Ca2+ entry. Suppression of inositol 1,4,5-trisphosphate formation by 2 μM U73122 (a phospholipase C inhibitor) did not affect 50 μM fluoxetine-induced Ca2+ release. Collectively, this study shows that fluoxetine increased [Ca2+]i in bladder cancer cells in a concentration-dependent fashion, by releasing Ca2+ from thapsigargin-sensitive Ca2+ stores in an IP3-independent manner, and by inducing Ca2+ influx from extracellular medium.

Original languageEnglish
Pages (from-to)503-507
Number of pages5
JournalPharmacological Research
Issue number5
StatePublished - 2001
Externally publishedYes


  • Antidepressant
  • Bladder cancer cells
  • Ca
  • Fluoxetine
  • Fura-2
  • Serotonin


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