Abstract
The effect of 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS), a presumed phospholipase C activator, on cytosolic free Ca 2+ concentrations ([Ca 2+ ] i ) in PC3 human prostate cancer cells is unclear. This study explored whether m-3M3FBS changed basal [Ca 2+ ] i levels in suspended PC3 cells by using fura-2 as a Ca 2+ -sensitive fluorescent dye. M-3M3FBS at concentrations between 10-50 μM increased [Ca 2+ ] i in a concentration-dependent manner. The Ca 2+ signal was reduced by 60% by removing extracellular Ca 2+ . M-3M3FBS-induced Ca 2+ influx was inhibited by the storeoperated Ca 2+ channel blockers nifedipine, econazole and SK&F96365, and by the phospholipase A2 inhibitor aristolochic acid. In Ca 2+ -free medium, 30 μM m-3M3FBS pretreatment greatly inhibited the [Ca 2+ ] i rise induced by the endoplasmic reticulum Ca 2+ pump inhibitor thapsigargin or BHQ. Conversely, pretreatment with thapsigargin, BHQ or cyclopiazonic acid reduced the major part of m-3M3FBSinduced [Ca 2+ ] i rise. Inhibition of phospholipase C with U73122 did not much alter m-3M3FBS-induced [Ca 2+ ] i rise. Collectively, in PC3 cells, m-3M3FBS induced [Ca 2+ ] i rises by causing phospholipase Cindependent Ca 2+ release from the endoplasmic reticulum and Ca 2+ influx via store-operated Ca 2+ channels.
Original language | English |
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Pages (from-to) | 151-159 |
Number of pages | 9 |
Journal | Chinese Journal of Physiology |
Volume | 53 |
Issue number | 3 |
DOIs | |
State | Published - 2010 |
Externally published | Yes |
Keywords
- PC3
- Prostate
- m-3M3FBS