TY - JOUR
T1 - Effect of relaxin and antirelaxin serum on porcine sperm motility
AU - Juang, H. H.
AU - Musah, A. I.
AU - Schwabe, C.
AU - Anderson, L. L.
PY - 1989/7
Y1 - 1989/7
N2 - Sperm motility was estimated by a transmembrane migration ratio (TMMR) method, which is based on the proportion of sperm that migrate across a membrane containing micropores with a diameter slightly greater than the width of the sperm nucleus. Relaxin (1000, 333 and 167 ng/ml) attenuated (P < 0.01) loss of sperm motility during 120 min (50 vs 40%). The addition of 1000 ng/ml relaxin to semen improved sperm motility (P < 0.05) stored at 12°C for 5 days with compared controls (43 vs 32%). Antirelaxin serum (R6) and normal rabbit serum (NRS; 1:9000) were added to semen and the sperm motility was determined at 0, 10, 40, 80 and 120 min. Antirelaxin serum abruptly inhibited sperm motility (P < 0.01) within 10 min compared with NRS-treated control semen (25 vs 58%). Caffeine and relaxin stimulated motility (P < 0.01) of frozen-thawed porcine semen; however, relaxin maintained greater sperm motility (P < 0.01) than caffeine after 80 min (30 vs 16%). These results indicate that purified porcine relaxin can maintain or increase sperm motility; in contrast, porcine antirelaxin abruptly inhibits sperm motility.
AB - Sperm motility was estimated by a transmembrane migration ratio (TMMR) method, which is based on the proportion of sperm that migrate across a membrane containing micropores with a diameter slightly greater than the width of the sperm nucleus. Relaxin (1000, 333 and 167 ng/ml) attenuated (P < 0.01) loss of sperm motility during 120 min (50 vs 40%). The addition of 1000 ng/ml relaxin to semen improved sperm motility (P < 0.05) stored at 12°C for 5 days with compared controls (43 vs 32%). Antirelaxin serum (R6) and normal rabbit serum (NRS; 1:9000) were added to semen and the sperm motility was determined at 0, 10, 40, 80 and 120 min. Antirelaxin serum abruptly inhibited sperm motility (P < 0.01) within 10 min compared with NRS-treated control semen (25 vs 58%). Caffeine and relaxin stimulated motility (P < 0.01) of frozen-thawed porcine semen; however, relaxin maintained greater sperm motility (P < 0.01) than caffeine after 80 min (30 vs 16%). These results indicate that purified porcine relaxin can maintain or increase sperm motility; in contrast, porcine antirelaxin abruptly inhibits sperm motility.
UR - https://www.scopus.com/pages/publications/38249023473
U2 - 10.1016/0378-4320(89)90110-3
DO - 10.1016/0378-4320(89)90110-3
M3 - 文章
AN - SCOPUS:38249023473
SN - 0378-4320
VL - 20
SP - 21
EP - 29
JO - Animal Reproduction Science
JF - Animal Reproduction Science
IS - 1
ER -