TY - JOUR
T1 - Effect of Three Clinical Therapies on Cytokines Modulation in the Hip Articular Cartilage and Bone Improvement in Rat Early Osteonecrosis of the Femoral Head.
AU - Hsu, SL
AU - Jhan, SW
AU - Hsu, Chih-Chin
AU - Wu, YN
AU - Wu, KL
AU - Kuo, CA
AU - Chiu, HW
AU - Cheng, JH
PY - 2022
Y1 - 2022
N2 - Extracorporeal shockwave therapy (ESWT) and adipose-derived mesenchymal stem cells (ADSCs) have been used clinically for the treatment of osteonecrosis of the femoral head (ONFH). The study elucidated that ESWT, ADSCs, and combination therapy modulated pro-inflammatory cytokines in the articular cartilage and subchondral bone of early rat ONFH.
ESWT and ADSCs were prepared and isolated for treatment. Micro-CT, pathological analysis, and immunohistochemistry were performed and analysed.
After treatments, subchondral bone of ONFH was improved in trabecular bone volume (BV/TV) (P < 0.001), thickness (Tb.Th) (P < 0.01 and 0.001), and separation (Tb.Sp) (P < 0.001) and bone mineral density (BMD) (P < 0.001) using micro-CT analysis. The articular cartilage was protected and decreased apoptosis markers after all the treatments. The expression of IL33 (P < 0.001), IL5 (P < 0.001), IL6 (P < 0.001), and IL17A (P < 0.01) was significantly decreased in the ESWT, ADSCs, and Combination groups as compared with ONFH group. The IL33 receptor ST2 was significantly increased after treatment (P < 0.001) as compared with ONFH group. The Combination group (P < 0.01) decreased the expression of IL6 better than the ESWT and ADSCs groups.
ESWT, ADSCs and combination therapy significantly protected articular cartilage and subchondral bone of early rat ONFH by modulating the expression of pro-inflammatory cytokines including, IL33 and its receptor ST2, IL5, IL6, and IL17A.
AB - Extracorporeal shockwave therapy (ESWT) and adipose-derived mesenchymal stem cells (ADSCs) have been used clinically for the treatment of osteonecrosis of the femoral head (ONFH). The study elucidated that ESWT, ADSCs, and combination therapy modulated pro-inflammatory cytokines in the articular cartilage and subchondral bone of early rat ONFH.
ESWT and ADSCs were prepared and isolated for treatment. Micro-CT, pathological analysis, and immunohistochemistry were performed and analysed.
After treatments, subchondral bone of ONFH was improved in trabecular bone volume (BV/TV) (P < 0.001), thickness (Tb.Th) (P < 0.01 and 0.001), and separation (Tb.Sp) (P < 0.001) and bone mineral density (BMD) (P < 0.001) using micro-CT analysis. The articular cartilage was protected and decreased apoptosis markers after all the treatments. The expression of IL33 (P < 0.001), IL5 (P < 0.001), IL6 (P < 0.001), and IL17A (P < 0.01) was significantly decreased in the ESWT, ADSCs, and Combination groups as compared with ONFH group. The IL33 receptor ST2 was significantly increased after treatment (P < 0.001) as compared with ONFH group. The Combination group (P < 0.01) decreased the expression of IL6 better than the ESWT and ADSCs groups.
ESWT, ADSCs and combination therapy significantly protected articular cartilage and subchondral bone of early rat ONFH by modulating the expression of pro-inflammatory cytokines including, IL33 and its receptor ST2, IL5, IL6, and IL17A.
U2 - 10.1016/j.bj.2022.11.004
DO - 10.1016/j.bj.2022.11.004
M3 - Journal Article
C2 - 36442793
SN - 2319-4170
VL - S2319-4170(22)00156-1
JO - Biomedical Journal
JF - Biomedical Journal
ER -