Effects of aging on erythropoietin secretion in female rats

Ray Yau Wang; Shiow Chwen Tsai; Chien Chen Lu; Yuh Fan Tung; Shyi Wu Wang; Paulus S. Wang

doi:10.1016/S0047-6374(98)00031-1

Effects of aging on erythropoietin secretion in female rats

Ray Yau Wang, Shiow Chwen Tsai, Chien Chen Lu, Yuh Fan Tung, Shyi Wu Wang, Paulus S. Wang^*

^*Corresponding author for this work

Department of Physiology

National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

The secretion of erythropoietin (EPO) in old (22-25 months), middle (mid)-aged (15-17 months), adult (6-7 months), and young (3 months) female rats was studied. Rats of different ages were bled by cardiac puncture for measuring plasma EPO, estradiol, and progesterone levels. The metabolic clearance rate of EPO was determined by a single-injection method. The effects of ovariectomy and replacement of estradiol (E) and/or progesterone (P) on plasma EPO concentration were also investigated. Analysis of direct effects of E and/or P on EPO from kidney tissues was carried out in an in vitro study. Plasma EPO levels were higher in both mid-aged and old rats than in young rats (P < 0.01). The metabolic clearance rate of EPO was also greater in adult, mid-aged and old rats than in young rats (P < 0.01). Ovariectomy increased rat plasma EPO concentration which might be inhibited by E (P < 0.01), P (P < 0.01), or E and P (P < 0.05) replacement. Neither E (10^-10 or 10^-9 M) nor P (10^-8 or 10^-7 M) alone altered EPO release from kidney tissue in vitro. Combination of E (10^-10 M) and P (10^-8 or 10^-7 M) stimulated the EPO release from kidney tissue (P < 0.01). These data suggest that the mechanism of the secretion of EPO by kidneys in female rats during aging is ovarian steroid hormones independent.

Original language	English
Pages (from-to)	81-90
Number of pages	10
Journal	Mechanisms of Ageing and Development
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/S0047-6374(98)00031-1
State	Published - 01 06 1998

Keywords

Aging
Erythropoietin
Estradiol
Female rats
Progesterone

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10.1016/S0047-6374(98)00031-1

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title = "Effects of aging on erythropoietin secretion in female rats",

abstract = "The secretion of erythropoietin (EPO) in old (22-25 months), middle (mid)-aged (15-17 months), adult (6-7 months), and young (3 months) female rats was studied. Rats of different ages were bled by cardiac puncture for measuring plasma EPO, estradiol, and progesterone levels. The metabolic clearance rate of EPO was determined by a single-injection method. The effects of ovariectomy and replacement of estradiol (E) and/or progesterone (P) on plasma EPO concentration were also investigated. Analysis of direct effects of E and/or P on EPO from kidney tissues was carried out in an in vitro study. Plasma EPO levels were higher in both mid-aged and old rats than in young rats (P < 0.01). The metabolic clearance rate of EPO was also greater in adult, mid-aged and old rats than in young rats (P < 0.01). Ovariectomy increased rat plasma EPO concentration which might be inhibited by E (P < 0.01), P (P < 0.01), or E and P (P < 0.05) replacement. Neither E (10-10 or 10-9 M) nor P (10-8 or 10-7 M) alone altered EPO release from kidney tissue in vitro. Combination of E (10-10 M) and P (10-8 or 10-7 M) stimulated the EPO release from kidney tissue (P < 0.01). These data suggest that the mechanism of the secretion of EPO by kidneys in female rats during aging is ovarian steroid hormones independent.",

keywords = "Aging, Erythropoietin, Estradiol, Female rats, Progesterone",

author = "Wang, {Ray Yau} and Tsai, {Shiow Chwen} and Lu, {Chien Chen} and Tung, {Yuh Fan} and Wang, {Shyi Wu} and Wang, {Paulus S.}",

year = "1998",

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T1 - Effects of aging on erythropoietin secretion in female rats

AU - Wang, Ray Yau

AU - Tsai, Shiow Chwen

AU - Lu, Chien Chen

AU - Tung, Yuh Fan

AU - Wang, Shyi Wu

AU - Wang, Paulus S.

PY - 1998/6/1

Y1 - 1998/6/1

N2 - The secretion of erythropoietin (EPO) in old (22-25 months), middle (mid)-aged (15-17 months), adult (6-7 months), and young (3 months) female rats was studied. Rats of different ages were bled by cardiac puncture for measuring plasma EPO, estradiol, and progesterone levels. The metabolic clearance rate of EPO was determined by a single-injection method. The effects of ovariectomy and replacement of estradiol (E) and/or progesterone (P) on plasma EPO concentration were also investigated. Analysis of direct effects of E and/or P on EPO from kidney tissues was carried out in an in vitro study. Plasma EPO levels were higher in both mid-aged and old rats than in young rats (P < 0.01). The metabolic clearance rate of EPO was also greater in adult, mid-aged and old rats than in young rats (P < 0.01). Ovariectomy increased rat plasma EPO concentration which might be inhibited by E (P < 0.01), P (P < 0.01), or E and P (P < 0.05) replacement. Neither E (10-10 or 10-9 M) nor P (10-8 or 10-7 M) alone altered EPO release from kidney tissue in vitro. Combination of E (10-10 M) and P (10-8 or 10-7 M) stimulated the EPO release from kidney tissue (P < 0.01). These data suggest that the mechanism of the secretion of EPO by kidneys in female rats during aging is ovarian steroid hormones independent.

AB - The secretion of erythropoietin (EPO) in old (22-25 months), middle (mid)-aged (15-17 months), adult (6-7 months), and young (3 months) female rats was studied. Rats of different ages were bled by cardiac puncture for measuring plasma EPO, estradiol, and progesterone levels. The metabolic clearance rate of EPO was determined by a single-injection method. The effects of ovariectomy and replacement of estradiol (E) and/or progesterone (P) on plasma EPO concentration were also investigated. Analysis of direct effects of E and/or P on EPO from kidney tissues was carried out in an in vitro study. Plasma EPO levels were higher in both mid-aged and old rats than in young rats (P < 0.01). The metabolic clearance rate of EPO was also greater in adult, mid-aged and old rats than in young rats (P < 0.01). Ovariectomy increased rat plasma EPO concentration which might be inhibited by E (P < 0.01), P (P < 0.01), or E and P (P < 0.05) replacement. Neither E (10-10 or 10-9 M) nor P (10-8 or 10-7 M) alone altered EPO release from kidney tissue in vitro. Combination of E (10-10 M) and P (10-8 or 10-7 M) stimulated the EPO release from kidney tissue (P < 0.01). These data suggest that the mechanism of the secretion of EPO by kidneys in female rats during aging is ovarian steroid hormones independent.

KW - Aging

KW - Erythropoietin

KW - Estradiol

KW - Female rats

KW - Progesterone

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VL - 103

SP - 81

EP - 90

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

IS - 1

ER -

Effects of aging on erythropoietin secretion in female rats

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Keywords

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