Effects of fibronectin on the interaction of polymorphonuclear leukocytes with unopsonized and antibody-opsonized bacteria

Kuender D. Yang, Nancy H. Augustine, Luis A. Gonzalez, John F. Bohnsack, Harry R. Hill*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

35 Scopus citations

Abstract

Fibronectin (Fn) affects the interaction of polymorphonuclear leukocytes (PMNLs) with certain bacteria. Fn alone enhanced the response, in a chemiluminescence (CL) assay, of PMNLs to Staphylococcus aureus (P <.05) and Staphylococcus epidermidis (P <.01) but had no effect on type III, group B streptococci (GBS) or Escherichia coli. When OBS or E. coli were first preopsonized in antibody, Fn significantly enhanced the CL response of PMNLs (P <.05). The intracellular metabolic inhibitor NaN3 but not the extracellular scavengers superoxide dismutase or human serum albumin inhibited Fn-enhanced CL; this fact suggests that enhancement of the respiratory burst by Fn is an intracellular event. We used an acridine orange-crystal violet monolayer assay to examine the effects of Fn on ingestion and intracellular killing of bacteria by PMNLs. Fn alone promoted uptake and killing of S. aureus (P <.01) and S. epidermidis (P <.05) by PMNLs but did not enhance monolayer phagocytosis of GBS or E. coli, unless these bacteria were preopsonized in antibody (P <.01).

Original languageEnglish
Pages (from-to)823-830
Number of pages8
JournalJournal of Infectious Diseases
Volume158
Issue number4
DOIs
StatePublished - 10 1988
Externally publishedYes

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