TY - JOUR
T1 - Effects of polyphenols derived from fruit of Crataegus pinnatifida on cell transformation, dermal edema and skin tumor formation by phorbol ester application
AU - Kao, Erl Shyh
AU - Wang, Chau Jong
AU - Lin, Wea Lung
AU - Chu, Chia Yih
AU - Tseng, Tsui Hwa
PY - 2007/10
Y1 - 2007/10
N2 - The dried fruits of Crataegus pinnatifida have been used traditionally as oriental medicine and local soft drink material recently. Previously, we demonstrated that C. pinnatifida exhibited anti-oxidation and anti-inflammatory potential. To clarify the active components in anti-transformation and anti-tumor promotion, we collected the polyphenol fraction (CF-TP) of hot-water extracts from dried fruits of C. pinnatifida for the following study. By anchorage-independent transformation assay, CF-TP significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in JB6 P+ cells. Moreover, we found that CF-TP inhibited the expression of osteopontin (OPN), a transformational marker, and the activation of NF-κB and AP-1 induced by TPA in JB6 P+ cells. In addition, we evaluated the effect of CF-TP on TPA application to ICR mouse skin with measurement of H2O2 production, myeloperoxidase (MPO) activity, edema formation, epidermal thickness and leukocyte infiltration. As a result, CF-TP significantly inhibited the generation of reactive oxygen species (ROS) and the phenomena of inflammation induced by TPA. It also suppressed the expression of COX-2 and iNOS, and the activation of ornithine decarboxylase (ODC). Furthermore, CF-TP inhibited benzo[a]pyrene (B[a]P)/TPA-induced skin tumor formation and decreased the incidence of tumor. These results indicate that CF-TP possesses potential as a cancer chemopreventive agent against tumor promotion.
AB - The dried fruits of Crataegus pinnatifida have been used traditionally as oriental medicine and local soft drink material recently. Previously, we demonstrated that C. pinnatifida exhibited anti-oxidation and anti-inflammatory potential. To clarify the active components in anti-transformation and anti-tumor promotion, we collected the polyphenol fraction (CF-TP) of hot-water extracts from dried fruits of C. pinnatifida for the following study. By anchorage-independent transformation assay, CF-TP significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in JB6 P+ cells. Moreover, we found that CF-TP inhibited the expression of osteopontin (OPN), a transformational marker, and the activation of NF-κB and AP-1 induced by TPA in JB6 P+ cells. In addition, we evaluated the effect of CF-TP on TPA application to ICR mouse skin with measurement of H2O2 production, myeloperoxidase (MPO) activity, edema formation, epidermal thickness and leukocyte infiltration. As a result, CF-TP significantly inhibited the generation of reactive oxygen species (ROS) and the phenomena of inflammation induced by TPA. It also suppressed the expression of COX-2 and iNOS, and the activation of ornithine decarboxylase (ODC). Furthermore, CF-TP inhibited benzo[a]pyrene (B[a]P)/TPA-induced skin tumor formation and decreased the incidence of tumor. These results indicate that CF-TP possesses potential as a cancer chemopreventive agent against tumor promotion.
KW - 12-O-Tetradecanoylphorbol-13-acetate
KW - Crataegus pinnatifida
KW - Osteopontin
KW - Skin tumor
KW - Transformation
UR - http://www.scopus.com/inward/record.url?scp=34547728645&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2007.03.016
DO - 10.1016/j.fct.2007.03.016
M3 - 文章
C2 - 17493734
AN - SCOPUS:34547728645
SN - 0278-6915
VL - 45
SP - 1795
EP - 1804
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 10
ER -
