Effects of rosiglitazone on global ischemia-induced hippocampal injury and expression of mitochondrial uncoupling protein 2

Shang Der Chen; Hsin Yi Wu; Ding I. Yang; Su Ying Lee; Fu Zen Shaw; Tsu Kung Lin; Chia Wei Liou; Yao Chung Chuang

doi:10.1016/j.bbrc.2006.10.017

Effects of rosiglitazone on global ischemia-induced hippocampal injury and expression of mitochondrial uncoupling protein 2

Shang Der Chen
, Hsin Yi Wu
, Ding I. Yang
, Su Ying Lee
, Fu Zen Shaw
, Tsu Kung Lin
, Chia Wei Liou
, Yao Chung Chuang^*

^*Corresponding author for this work

Chang Gung University
National Sun Yat-sen University
National Yang Ming Chiao Tung University
National Cheng Kung University

Research output: Contribution to journal › Journal Article › peer-review

49 Scopus citations

Abstract

We investigate the effect of rosiglitazone, a ligand for peroxisome proliferator-activated receptor-γ (PPARγ) with anti-inflammatory and anti-oxidative actions, on hippocampal injury and its roles in mitochondrial uncoupling protein 2 (UCP2) expression caused by transient global ischemia (TGI) in rats. Increased UCP2 expression was observed in mitochondria of hippocampal CA1 2-24 h after TGI/reperfusion, with maximal expression levels at 6-18 h. Administration of rosiglitazone to hippocampus 30 min prior to the onset of TGI further enhanced mitochondrial UCP2 expression 2-6 h following TGI/reperfusion. Rats subjected to TGI/reperfusion displayed a significant increase in lipid peroxidation, based on increased malondialdehyde (MDA) levels, in hippocampal CA1 mitochondria 2-6 h after reperfusion. Rosiglitazone significantly attenuated TGI/reperfusion-induced lipid peroxidation and suppressed hippocampal CA1 neuronal death based on the surviving neuronal counts. In conclusion, our results provide correlative evidence for the "PPARγ → UCP2 → neuroprotection" cascade in ischemic brain injury.

Original language	English
Pages (from-to)	198-203
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	351
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.10.017
State	Published - 08 12 2006
Externally published	Yes

Keywords

Global ischemia
Hippocampus
Mitochondria
Peroxisome proliferator-activated receptor-γ
Rosiglitazone
Uncoupling protein 2

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title = "Effects of rosiglitazone on global ischemia-induced hippocampal injury and expression of mitochondrial uncoupling protein 2",

abstract = "We investigate the effect of rosiglitazone, a ligand for peroxisome proliferator-activated receptor-γ (PPARγ) with anti-inflammatory and anti-oxidative actions, on hippocampal injury and its roles in mitochondrial uncoupling protein 2 (UCP2) expression caused by transient global ischemia (TGI) in rats. Increased UCP2 expression was observed in mitochondria of hippocampal CA1 2-24 h after TGI/reperfusion, with maximal expression levels at 6-18 h. Administration of rosiglitazone to hippocampus 30 min prior to the onset of TGI further enhanced mitochondrial UCP2 expression 2-6 h following TGI/reperfusion. Rats subjected to TGI/reperfusion displayed a significant increase in lipid peroxidation, based on increased malondialdehyde (MDA) levels, in hippocampal CA1 mitochondria 2-6 h after reperfusion. Rosiglitazone significantly attenuated TGI/reperfusion-induced lipid peroxidation and suppressed hippocampal CA1 neuronal death based on the surviving neuronal counts. In conclusion, our results provide correlative evidence for the {"}PPARγ → UCP2 → neuroprotection{"} cascade in ischemic brain injury.",

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author = "Chen, \{Shang Der\} and Wu, \{Hsin Yi\} and Yang, \{Ding I.\} and Lee, \{Su Ying\} and Shaw, \{Fu Zen\} and Lin, \{Tsu Kung\} and Liou, \{Chia Wei\} and Chuang, \{Yao Chung\}",

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AU - Chen, Shang Der

AU - Wu, Hsin Yi

AU - Yang, Ding I.

AU - Lee, Su Ying

AU - Shaw, Fu Zen

AU - Lin, Tsu Kung

AU - Liou, Chia Wei

AU - Chuang, Yao Chung

PY - 2006/12/8

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N2 - We investigate the effect of rosiglitazone, a ligand for peroxisome proliferator-activated receptor-γ (PPARγ) with anti-inflammatory and anti-oxidative actions, on hippocampal injury and its roles in mitochondrial uncoupling protein 2 (UCP2) expression caused by transient global ischemia (TGI) in rats. Increased UCP2 expression was observed in mitochondria of hippocampal CA1 2-24 h after TGI/reperfusion, with maximal expression levels at 6-18 h. Administration of rosiglitazone to hippocampus 30 min prior to the onset of TGI further enhanced mitochondrial UCP2 expression 2-6 h following TGI/reperfusion. Rats subjected to TGI/reperfusion displayed a significant increase in lipid peroxidation, based on increased malondialdehyde (MDA) levels, in hippocampal CA1 mitochondria 2-6 h after reperfusion. Rosiglitazone significantly attenuated TGI/reperfusion-induced lipid peroxidation and suppressed hippocampal CA1 neuronal death based on the surviving neuronal counts. In conclusion, our results provide correlative evidence for the "PPARγ → UCP2 → neuroprotection" cascade in ischemic brain injury.

AB - We investigate the effect of rosiglitazone, a ligand for peroxisome proliferator-activated receptor-γ (PPARγ) with anti-inflammatory and anti-oxidative actions, on hippocampal injury and its roles in mitochondrial uncoupling protein 2 (UCP2) expression caused by transient global ischemia (TGI) in rats. Increased UCP2 expression was observed in mitochondria of hippocampal CA1 2-24 h after TGI/reperfusion, with maximal expression levels at 6-18 h. Administration of rosiglitazone to hippocampus 30 min prior to the onset of TGI further enhanced mitochondrial UCP2 expression 2-6 h following TGI/reperfusion. Rats subjected to TGI/reperfusion displayed a significant increase in lipid peroxidation, based on increased malondialdehyde (MDA) levels, in hippocampal CA1 mitochondria 2-6 h after reperfusion. Rosiglitazone significantly attenuated TGI/reperfusion-induced lipid peroxidation and suppressed hippocampal CA1 neuronal death based on the surviving neuronal counts. In conclusion, our results provide correlative evidence for the "PPARγ → UCP2 → neuroprotection" cascade in ischemic brain injury.

KW - Global ischemia

KW - Hippocampus

KW - Mitochondria

KW - Peroxisome proliferator-activated receptor-γ

KW - Rosiglitazone

KW - Uncoupling protein 2

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JF - Biochemical and Biophysical Research Communications

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ER -

Effects of rosiglitazone on global ischemia-induced hippocampal injury and expression of mitochondrial uncoupling protein 2

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