TY - JOUR
T1 - Efficacy of antimicrobial peptides (AMPs) against Escherichia coli and bacteria morphology change after AMP exposure
AU - Huang, Yu Tzu
AU - Kumar, Selvaraj Rajesh
AU - Chan, Hao Chun
AU - Jhan, Zih Huei
AU - Chen, Dave W.
AU - Lue, Shingjiang Jessie
N1 - Publisher Copyright:
© 2021 Taiwan Institute of Chemical Engineers
PY - 2021/9
Y1 - 2021/9
N2 - In recent years, bacterial drug resistance has become a major health concern worldwide owing to the limited efficacy of some traditional antibiotics after long-term usage. Here, to search for potent bacteriostatic agents, six cationic antimicrobial peptides (AMPs) were investigated. L-GL13K, D-GL13K, MSI-594, indolicidin, Ib-M6 and nisinA-S29A were selected to study their bactericidal efficiency and the antibacterial mechanisms against Escherichia coli (E. coli). In addition to half-maximal inhibitory concentration (IC50) and minimum inhibitory concentration (MIC) determinations using the standard method, the initial bacterial growth profiles at 4 h of AMP exposure were used to study the possible antibacterial mechanisms. The AMP-treated E. coli exhibited collapsed cell walls and various nanosized and microsized pores on the bacterial surface, which led to transmembrane channels and pore formation. Among the studied peptides, MSI-594, D-GL13K, and L-GL13K (in decreasing order) were identified as superior bactericidal agents, which may be attributed to the suitable conformational structure and surface charge during the interaction between these AMPs and bacteria.
AB - In recent years, bacterial drug resistance has become a major health concern worldwide owing to the limited efficacy of some traditional antibiotics after long-term usage. Here, to search for potent bacteriostatic agents, six cationic antimicrobial peptides (AMPs) were investigated. L-GL13K, D-GL13K, MSI-594, indolicidin, Ib-M6 and nisinA-S29A were selected to study their bactericidal efficiency and the antibacterial mechanisms against Escherichia coli (E. coli). In addition to half-maximal inhibitory concentration (IC50) and minimum inhibitory concentration (MIC) determinations using the standard method, the initial bacterial growth profiles at 4 h of AMP exposure were used to study the possible antibacterial mechanisms. The AMP-treated E. coli exhibited collapsed cell walls and various nanosized and microsized pores on the bacterial surface, which led to transmembrane channels and pore formation. Among the studied peptides, MSI-594, D-GL13K, and L-GL13K (in decreasing order) were identified as superior bactericidal agents, which may be attributed to the suitable conformational structure and surface charge during the interaction between these AMPs and bacteria.
KW - Antimicrobial peptides
KW - Bactericidal mechanism
KW - Gram-negative bacteria
KW - Inhibitory concentration
KW - Transmembrane pores
UR - http://www.scopus.com/inward/record.url?scp=85110674584&partnerID=8YFLogxK
U2 - 10.1016/j.jtice.2021.07.003
DO - 10.1016/j.jtice.2021.07.003
M3 - 文章
AN - SCOPUS:85110674584
SN - 1876-1070
VL - 126
SP - 307
EP - 312
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
ER -