TY - GEN
T1 - Electrical detection method of protein concentration on ITO interdigitated electrodes
AU - Lei, Kin Fong
PY - 2011
Y1 - 2011
N2 - An electrical detection method of the concentration of the protein immobilizing on a biochip has been developed based on the indium tin oxide (ITO) interdigitated electrodes as the detection sites. The result of the protein concentration can be measured by a multimeter directly. The proposed protocol was to immobilize protein on the ITO interdigitated electrodes on a glass substrate. The immobilized protein was prepared to conjugate to biotin. Then, gold nanoparticles were bound to the protein through biotin-streptavidin linkage. Gold enhancement was performed for enlarging the gold nanoparticles. Therefore, a conductive layer was formed across the electrode gaps. The resistance values across the electrode can be measured by a multimeter directly. From the result, the concentrations of the immobilized protein between 5 and 1000 ng/ml can be represented by the resistance values quantitatively. The proposed detection method is simplicity, low cost, and miniaturization, therefore, it is possible to be integrated into the microfluidic system and potentially be developed a portable diagnostic device.
AB - An electrical detection method of the concentration of the protein immobilizing on a biochip has been developed based on the indium tin oxide (ITO) interdigitated electrodes as the detection sites. The result of the protein concentration can be measured by a multimeter directly. The proposed protocol was to immobilize protein on the ITO interdigitated electrodes on a glass substrate. The immobilized protein was prepared to conjugate to biotin. Then, gold nanoparticles were bound to the protein through biotin-streptavidin linkage. Gold enhancement was performed for enlarging the gold nanoparticles. Therefore, a conductive layer was formed across the electrode gaps. The resistance values across the electrode can be measured by a multimeter directly. From the result, the concentrations of the immobilized protein between 5 and 1000 ng/ml can be represented by the resistance values quantitatively. The proposed detection method is simplicity, low cost, and miniaturization, therefore, it is possible to be integrated into the microfluidic system and potentially be developed a portable diagnostic device.
KW - Biochip
KW - Electrical immunoassay
KW - Microfluidics
KW - Nanogold
UR - http://www.scopus.com/inward/record.url?scp=80053329512&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2011.6017529
DO - 10.1109/NEMS.2011.6017529
M3 - 会议稿件
AN - SCOPUS:80053329512
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 1020
EP - 1023
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -