Electrical detection method of protein concentration on ITO interdigitated electrodes

Kin Fong Lei*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

An electrical detection method of the concentration of the protein immobilizing on a biochip has been developed based on the indium tin oxide (ITO) interdigitated electrodes as the detection sites. The result of the protein concentration can be measured by a multimeter directly. The proposed protocol was to immobilize protein on the ITO interdigitated electrodes on a glass substrate. The immobilized protein was prepared to conjugate to biotin. Then, gold nanoparticles were bound to the protein through biotin-streptavidin linkage. Gold enhancement was performed for enlarging the gold nanoparticles. Therefore, a conductive layer was formed across the electrode gaps. The resistance values across the electrode can be measured by a multimeter directly. From the result, the concentrations of the immobilized protein between 5 and 1000 ng/ml can be represented by the resistance values quantitatively. The proposed detection method is simplicity, low cost, and miniaturization, therefore, it is possible to be integrated into the microfluidic system and potentially be developed a portable diagnostic device.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages1020-1023
Number of pages4
DOIs
StatePublished - 2011
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 20 02 201123 02 2011

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Conference

Conference6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Country/TerritoryTaiwan
CityKaohsiung
Period20/02/1123/02/11

Keywords

  • Biochip
  • Electrical immunoassay
  • Microfluidics
  • Nanogold

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