TY - JOUR
T1 - Electrochemical detection of cystatin C by oriented antibody immobilization on streptococcal protein G–modified ZIF-8-Cu1−xNix(OH)2@Cu core-shell nanostructured electrode
AU - Chakraborty, T.
AU - Das, M.
AU - Lin, C. Y.
AU - Kao, C. H.
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2023/1
Y1 - 2023/1
N2 - Herein, we prepared a novel nanostructure involving Cu shell on Zeolitic imidazolate frameworks (ZIF-8) and Cu1−xNix(OH)2 composite (ZIF-8-Cu1−xNix(OH)2@Cu) combining sol-gel and co-precipitation method. The morphology, stoichiometry, and structure of the nanocomposite were elucidated by various physicochemical analyses. A poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated indium tin oxide (ITO) was modified with the synthesized ZIF-8-Cu1−xNix(OH)2@Cu nanoparticles (NPs) to obtain an efficient electrode for further antibody immobilization. The ZIF-8-Cu1−xNix(OH)2@Cu/PEDOT:PSS/ITO was applied for the detection of cystatin C, a promising biomarker of chronic kidney disease (CKD). The electrode was functionalized by streptococcal protein G (SPG) to bind the Fc region of anti-cystatin C in an oriented manner. The synergistic catalytic activities, high surface coverage, enhanced electroactive sites, and excellent redox properties of the proposed electrode lead to excellent electrochemical sensing. The proposed sensor obtained a much lower detection limit (33 pg/mL) for a linear range of 0.1 ng/mL to 1,000 ng/mL with high selectivity, stability, and reproducibility compared with bare ZIF-8/PEDOT:PSS/ITO-based immunosensor. The clinical feasibility of the sensor was confirmed by measuring the human serum in the presence of different concentrations of cystatin C. This work demonstrates a new and facile approach to fabricating a metal-organic framework (MOF) –based nanoimmunosensor for cystatin C, which has significant importance in diagnosing the renal failure.
AB - Herein, we prepared a novel nanostructure involving Cu shell on Zeolitic imidazolate frameworks (ZIF-8) and Cu1−xNix(OH)2 composite (ZIF-8-Cu1−xNix(OH)2@Cu) combining sol-gel and co-precipitation method. The morphology, stoichiometry, and structure of the nanocomposite were elucidated by various physicochemical analyses. A poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coated indium tin oxide (ITO) was modified with the synthesized ZIF-8-Cu1−xNix(OH)2@Cu nanoparticles (NPs) to obtain an efficient electrode for further antibody immobilization. The ZIF-8-Cu1−xNix(OH)2@Cu/PEDOT:PSS/ITO was applied for the detection of cystatin C, a promising biomarker of chronic kidney disease (CKD). The electrode was functionalized by streptococcal protein G (SPG) to bind the Fc region of anti-cystatin C in an oriented manner. The synergistic catalytic activities, high surface coverage, enhanced electroactive sites, and excellent redox properties of the proposed electrode lead to excellent electrochemical sensing. The proposed sensor obtained a much lower detection limit (33 pg/mL) for a linear range of 0.1 ng/mL to 1,000 ng/mL with high selectivity, stability, and reproducibility compared with bare ZIF-8/PEDOT:PSS/ITO-based immunosensor. The clinical feasibility of the sensor was confirmed by measuring the human serum in the presence of different concentrations of cystatin C. This work demonstrates a new and facile approach to fabricating a metal-organic framework (MOF) –based nanoimmunosensor for cystatin C, which has significant importance in diagnosing the renal failure.
KW - CKD
KW - Cystatin C
KW - Immunosensor
KW - Zeolitic imidazolate frameworks
UR - http://www.scopus.com/inward/record.url?scp=85142683993&partnerID=8YFLogxK
U2 - 10.1016/j.mtchem.2022.101273
DO - 10.1016/j.mtchem.2022.101273
M3 - 文章
AN - SCOPUS:85142683993
SN - 2468-5194
VL - 27
JO - Materials Today Chemistry
JF - Materials Today Chemistry
M1 - 101273
ER -
