Electrospun nanofibrous thermoplastic polyurethane/poly(glycerol sebacate) hybrid scaffolds for vocal fold tissue engineering applications

Vocal fold tissue engineering requires biomimetic scaffolds with an appropriate matrix stiffness closely matching that of the natural vocal folds to maintain function. Traditionally, poly(ɛ‑caprolactone) (PCL) and thermoplastic polyurethane (TPU) have been employed as the primary matrix materials for vocal fold electrospun scaffolds. However, not all of the scaffolds fabricated thus far matched the human vocal fold tissues. Poly(glycerol sebacate) (PGS) is a non-cytotoxic and biodegradable soft elastomer that has shown promising results for soft tissue engineering applications. However, no work has been done to employ this biomaterial to construct vocal fold scaffolds. In this study, PGS has been synthesized and blended with thermoplastic polyurethane (TPU) to produce vocal fold scaffolds with improved hydrophilicity and compliance by electrospinning. The resulting scaffolds were found to have mechanical properties mimicking those of the vocal fold lamina propria extracellular matrix (ECM). An unusual leaf-like structure was obtained when using 1,1,1,3,3,3‑hexafluoroisopropanol (HFIP) as the solvent. Other suitable fibrous scaffolds were also obtained when using acetic acid and 2,2,2‑trifluoroethanol (TFE) as binary solvents. A biological evaluation of these TPU/PGS scaffolds showed better cell spreading and significantly improved cell proliferation as compared to TPU-only scaffolds (p < 0.01), thereby suggesting potential applications for vocal fold tissue engineering.