Electrospun polylactic acid (PLLA) microtube array membrane (MTAM)-An advanced substrate for anticancer drug screening

Chia Hsuan Tseng, Wan Ting Huang, Chee Ho Chew, Jun Kai Lai, Shih Hsin Tu, Po Li Wei, Kang Yun Lee, Gi Ming Lai, Chien Chung Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

12 Scopus citations


The advent of personalized cancer treatment resulted in the shift from the administration of cytotoxic drugs with broad activity spectrum to a targeted tumor-specific therapy. Aligned to this development, the focus of this study revolved around the application of our novel and patented microtube array membrane (MTAM) in the US National Cancer Institute (NCI) developed an HFA (hollow fiber assay) assay; hereinafter known as MTAM/HFA. Electrospun poly-L-lactic acid (PLLA) MTAM was sterilized and loaded with cell lines/patient derived tumor cells (PDTC) and subcutaneously implanted into the backs of BALB/C mice. Anticancer drugs were administered at the respective time points and the respective MTAMs were retrieved and the viability tumor cells within were quantified with the MTT assay. Results revealed that the MTAMs were excellent culture substrate for various cancer cell lines and PDTCs (patient derived tumor cells). Compared to traditional HFA systems that utilize traditional hollow fibers, MTAM/HFA revealed superior drug sensitivity for a wide range of anticancer drug classes. Additionally, the duration for each test was < 14 days; all this while capable of producing similar trend outcome to the current gold-standard xenograft models. These benefits were observed in both the in vitro and in vivo stages, making it a highly practical phenotypic-based solution that could potentially be applied in personalized medicine.

Original languageEnglish
Article number569
Issue number4
StatePublished - 14 02 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 by the authors.


  • Anticancer drug screening
  • Electrospinning
  • Hollow fiber assay (HFA)
  • Microtube array membrane (MTAM)
  • Personalized medicine


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