Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

Hsieh Fu Tsai, Shih Wei Peng, Chun Ying Wu, Hui Fang Chang, Ji Yen Cheng*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

26 Scopus citations


We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0).

Original languageEnglish
Article number034116
Issue number3
StatePublished - 18 07 2012
Externally publishedYes


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