Elevated amniotic fluid F2-isoprostane: A potential predictive marker for preeclampsia

Chao Nin Wang, Jannie Ying Syuan Chen, Sahadevan Sabu, Yao Lung Chang, Shuenn Dyh Chang, Chuan Chi Kao, Hsiu Huei Peng, Ho Yen Chueh, An Shine Chao, Po Jen Cheng, Yun Shien Lee*, Lang Ming Chi, Tzu Hao Wang

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

20 Scopus citations


In the complex mechanism of preeclampsia, oxidative stress is an important pathogenic factor, and F2-isoprostane is a marker of oxidative stress and lipid peroxidation. The objective of this study was to identify if the amniotic fluid (AF) levels of F2-isoprostanes were elevated in women who later developed preeclampsia. In this study, we analyzed AF F 2-isoprostane concentrations with enzyme immunoassay (EIA), and the EIA results could be validated by quantitative mass spectrometry. The mean AF concentration of F2-isoprostanes was significantly higher in pregnancies with subsequent development of preeclampsia (123.1 ± 57.6 pg/ml, n = 85) than in controls (73.8 ± 36.6 pg/ml, n = 85). The AF elevation of F2-isoprostanes was even higher in the preeclampsia with intrauterine growth restriction group (138.3 ± 65.2 pg/ml, n = 39). The area under the curve of the receiver operating characteristics analysis for AF F2-isoprostanes assay was 0.81, supporting its potential as a biomarker for preeclampsia. These results indicate that oxidative stress existed before the onset of clinical preeclampsia, further suggesting that the elevation of AF F2-isoprostanes may be used as a guide for antioxidant supplementation to reduce the risk and/or severity of preeclampsia.

Original languageEnglish
Pages (from-to)1124-1130
Number of pages7
JournalFree Radical Biology and Medicine
Issue number9
StatePublished - 01 05 2011
Externally publishedYes


  • Amniotic fluid
  • Fetal growth restriction
  • Free radical
  • Isoprostane
  • LC/MS
  • Oxidative stress
  • Preeclampsia


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