Enhanced expression of various exogenous genes in recombinant chinese hamster ovary cells in presence of dimethyl sulfoxide

Chi Hsien Liu; I. Ming Chu; Shiaw Min Hwang

doi:10.1023/A:1012466112116

Enhanced expression of various exogenous genes in recombinant chinese hamster ovary cells in presence of dimethyl sulfoxide

Chi Hsien Liu, I. Ming Chu^*, Shiaw Min Hwang

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

26 Scopus citations

Abstract

Dimethyl sulfoxide (DMSO) (1% v/v) stimulated stable transformed Chinese hamster ovary (CHO) cells to synthesize different recombinant proteins and repress their proliferation rate. The expressions of a fusion protein and β-galactosidase were increased 1.6- and 1.4-fold after adding DMSO. The expression of fusion protein was increased by up to 2.8-fold that of uninduced control by the simultaneous addition of DMSO and pentanoic acid. However, DMSO did not increase the production of the monoclonal antibody (immunoglobulin G) of three hybridoma cell lines (OKM1, OKT4 and HyGPD-YK-1-1), although it could inhibit the growth rates of the hybridoma.

Original language	English
Pages (from-to)	1641-1645
Number of pages	5
Journal	Biotechnology Letters
Volume	23
Issue number	20
DOIs	https://doi.org/10.1023/A:1012466112116
State	Published - 2001
Externally published	Yes

Keywords

Dimethyl sulfoxide
Gene expression
Recombinant mammalian cells

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title = "Enhanced expression of various exogenous genes in recombinant chinese hamster ovary cells in presence of dimethyl sulfoxide",

abstract = "Dimethyl sulfoxide (DMSO) (1% v/v) stimulated stable transformed Chinese hamster ovary (CHO) cells to synthesize different recombinant proteins and repress their proliferation rate. The expressions of a fusion protein and β-galactosidase were increased 1.6- and 1.4-fold after adding DMSO. The expression of fusion protein was increased by up to 2.8-fold that of uninduced control by the simultaneous addition of DMSO and pentanoic acid. However, DMSO did not increase the production of the monoclonal antibody (immunoglobulin G) of three hybridoma cell lines (OKM1, OKT4 and HyGPD-YK-1-1), although it could inhibit the growth rates of the hybridoma.",

keywords = "Dimethyl sulfoxide, Gene expression, Recombinant mammalian cells",

author = "Liu, {Chi Hsien} and Chu, {I. Ming} and Hwang, {Shiaw Min}",

year = "2001",

doi = "10.1023/A:1012466112116",

language = "英语",

volume = "23",

pages = "1641--1645",

journal = "Biotechnology Letters",

issn = "0141-5492",

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TY - JOUR

T1 - Enhanced expression of various exogenous genes in recombinant chinese hamster ovary cells in presence of dimethyl sulfoxide

AU - Liu, Chi Hsien

AU - Chu, I. Ming

AU - Hwang, Shiaw Min

PY - 2001

Y1 - 2001

N2 - Dimethyl sulfoxide (DMSO) (1% v/v) stimulated stable transformed Chinese hamster ovary (CHO) cells to synthesize different recombinant proteins and repress their proliferation rate. The expressions of a fusion protein and β-galactosidase were increased 1.6- and 1.4-fold after adding DMSO. The expression of fusion protein was increased by up to 2.8-fold that of uninduced control by the simultaneous addition of DMSO and pentanoic acid. However, DMSO did not increase the production of the monoclonal antibody (immunoglobulin G) of three hybridoma cell lines (OKM1, OKT4 and HyGPD-YK-1-1), although it could inhibit the growth rates of the hybridoma.

AB - Dimethyl sulfoxide (DMSO) (1% v/v) stimulated stable transformed Chinese hamster ovary (CHO) cells to synthesize different recombinant proteins and repress their proliferation rate. The expressions of a fusion protein and β-galactosidase were increased 1.6- and 1.4-fold after adding DMSO. The expression of fusion protein was increased by up to 2.8-fold that of uninduced control by the simultaneous addition of DMSO and pentanoic acid. However, DMSO did not increase the production of the monoclonal antibody (immunoglobulin G) of three hybridoma cell lines (OKM1, OKT4 and HyGPD-YK-1-1), although it could inhibit the growth rates of the hybridoma.

KW - Dimethyl sulfoxide

KW - Gene expression

KW - Recombinant mammalian cells

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DO - 10.1023/A:1012466112116

M3 - 文章

AN - SCOPUS:0034752965

SN - 0141-5492

VL - 23

SP - 1641

EP - 1645

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 20

ER -

Enhanced expression of various exogenous genes in recombinant chinese hamster ovary cells in presence of dimethyl sulfoxide

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