Enhanced therapeutic epidermal growth factor receptor (EGFR) antibody delivery via pulsed ultrasound with targeting microbubbles for glioma treatment

Ai Ho Liao, Hsin Yi Chou, Yi Lei Hsieh, Sheng Chieh Hsu, Kuo Chen Wei, Hao Li Liu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

13 Scopus citations

Abstract

Pulsed-mode ultrasound (pUS) in combination with intravenously (IV) administered microbubbles (MBs) can enhance local drug delivery by temporarily enhancing capillary permeability. This study evaluates the use of epidermal growth factor receptor (EGFR)-targeting MBs after pUS treatment to enhance the effects of therapeutic- EGFR antibody delivery to glioma tumor cells in mice. Three animal groups were compared: (1) IV-injected nontargeting MBs, (2) IV-injected targeting MBs, and (3) IVinjected targeting MBs combined with pUS treatment. All animals were analyzed using high-frequency small-animal US imaging. The mean halftime of circulating targeting MBs was significantly increased from 3.13 min of targeting bubble alone to 5.86 min by targeting MBs combined with pUS treatment, compared to 2.34 min for non-targeting MBs. Compared to targeting bubble administration alone, pUS exposure prior to injection of targeting MBs was also significantly better at suppressing tumor growth when monitored for up to 35 days (p<0.05). The final relative tumor volumes were 2664, 700, and 188 mm3 for non-targeting MBs, targeting MBs, and targeting MBs combined with pUS treatment, respectively. pUS treatment prolonged the mean circulatory halftime of targeting MBs and enhanced the anti-tumor effect of EGFR antibodies in a human glioma model in mice. Targeting MBs combined with pUS treatment thus has potential for enhanced therapeutic antibody delivery for facilitating anti-glioma treatment.

Original languageEnglish
Pages (from-to)156-164
Number of pages9
JournalJournal of Medical and Biological Engineering
Volume35
Issue number2
DOIs
StatePublished - 04 2015

Bibliographical note

Publisher Copyright:
© The Author(s) 2015.

Keywords

  • Epidermal growth factor receptor
  • Microbubbles
  • Pulsed ultrasound

Fingerprint

Dive into the research topics of 'Enhanced therapeutic epidermal growth factor receptor (EGFR) antibody delivery via pulsed ultrasound with targeting microbubbles for glioma treatment'. Together they form a unique fingerprint.

Cite this