Abstract
Cisplatin is an anticancer drug that can induce apoptosis. In this study, we investigated the effect of mitochondrial DNA (mtDNA) depletion on cisplatin-induced cell death using a human osteosarcoma cell line (143B) and mtDNA-depleted 143B cells (143B-p°). Results showed that cisplatin decreased cell survival in 143B-p° cells. Moreover, cisplatin induced a greater extent of apoptosis-associated DNA fragmentation and caspase 3 activation in 143B-p° cells. The release of mitochondrial cytochrome c into cytosol by cisplatin was enhanced more obviously in 143B cells than in 143B-p° cells; however, in the control group of 143B-p cells, it was already dramatically greater. Depletion of mtDNA may increase sensitivity of cells to cisplatin-induced apoptosis by enhancing caspase 3 activation via both cytochrome c-dependent and -independent pathways.
Original language | English |
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Pages (from-to) | 516-522 |
Number of pages | 7 |
Journal | Annals of the New York Academy of Sciences |
Volume | 1042 |
DOIs | |
State | Published - 2005 |
Keywords
- Apoptosis
- Caspase 3
- Cisplatin
- Cytochrome c
- Mitochondrial DNA