Enhancement of diabetic wound repair using biodegradable nanofibrous metformin-eluting membranes: In vitro and in vivo

Cheng Hung Lee, Ming Jer Hsieh, Shang Hung Chang, Yu Huang Lin, Shih Jung Liu*, Tzu Yu Lin, Kuo Chun Hung, Jong Hwei S. Pang, Jyuhn Huarng Juang

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

76 Scopus citations

Abstract

This work developed biodegradable nanofibrous drug-eluting membranes that provided sustained release of metformin for repairing wounds associated with diabetes. To prepare the biodegradable membranes, poly-d-l-lactide-glycolide (PLGA) and metformin were first dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and were spun into nanofibrous membranes by electrospinning. An elution method and an HPLC assay were utilized to characterize the in vivo and in vitro release rates of the pharmaceuticals from the membranes. The biodegradable nanofibrous membranes released high concentrations of metformin for more than three weeks. Moreover, nanofibrous metformin-eluting PLGA membranes were more hydrophilic and had a greater water-containing capacity than virgin PLGA fibers. The membranes also improved wound healing and re-epithelialization in diabetic rats relative to the control. The experimental results in this work suggest that nanofibrous metformin-eluting membranes were functionally active in the treatment of diabetic wounds and very effective as accelerators in the early stage of healing of such wounds.

Original languageEnglish
Pages (from-to)3979-3986
Number of pages8
JournalACS Applied Materials and Interfaces
Volume6
Issue number6
DOIs
StatePublished - 26 03 2014

Keywords

  • diabetic wound repair
  • electrospinning
  • nanofibrous metformin-eluting membranes
  • release characteristics

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