TY - JOUR
T1 - Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols
AU - Chang, Chia Chen
AU - Hsu, Tsz Lian
AU - Chen, Chie Pein
AU - Chen, Chen Yu
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/9
Y1 - 2020/9
N2 - A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 µM), with a detection limit of 0.5 µM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.
AB - A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 µM), with a detection limit of 0.5 µM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.
KW - Catalytic gold nanoparticle
KW - Colorimetric assay
KW - Iodine
KW - Peroxidase-like activity
UR - http://www.scopus.com/inward/record.url?scp=85090321006&partnerID=8YFLogxK
U2 - 10.3390/bios10090113
DO - 10.3390/bios10090113
M3 - 文章
C2 - 32882936
AN - SCOPUS:85090321006
SN - 2079-6374
VL - 10
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 113
ER -