Abstract
A phenomenological model describing the transfer of lysozyme between a bulk aqueous phase and a reversed micellar phase in a stirred membrane cell has been confirmed. Transport of the enzyme at the interface at low surfactant concentrations is dominant, while that through the membrane is the rate-determining step at high surfactant concentrations. Complete separation of a-chymotrypsin from lysozyme using a supported liquid membrane filled with reversed micelles demonstrates the feasibility of the present process for enzyme separation.
Original language | English |
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Pages (from-to) | 2551-2563 |
Number of pages | 13 |
Journal | Separation Science and Technology |
Volume | 30 |
Issue number | 12 |
DOIs | |
State | Published - 01 07 1995 |
Externally published | Yes |
Keywords
- . Reversed micelles
- Enzyme separation
- Supported liquid membrane