Enzyme Separation Using Supported Liquid Membrane Filled with Reversed Micelles

Shau Wei Tsai, Jun Lone Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

1 Scopus citations

Abstract

A phenomenological model describing the transfer of lysozyme between a bulk aqueous phase and a reversed micellar phase in a stirred membrane cell has been confirmed. Transport of the enzyme at the interface at low surfactant concentrations is dominant, while that through the membrane is the rate-determining step at high surfactant concentrations. Complete separation of a-chymotrypsin from lysozyme using a supported liquid membrane filled with reversed micelles demonstrates the feasibility of the present process for enzyme separation.

Original languageEnglish
Pages (from-to)2551-2563
Number of pages13
JournalSeparation Science and Technology
Volume30
Issue number12
DOIs
StatePublished - 01 07 1995
Externally publishedYes

Keywords

  • . Reversed micelles
  • Enzyme separation
  • Supported liquid membrane

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