Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease

Mindy Ming Huey Guo, Ling Sai Chang, Ying Hsien Huang, Feng Sheng Wang, Ho Chang Kuo*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

24 Scopus citations

Abstract

Kawasaki disease (KD) is a common systemic vasculitides in children younger than 5 years of age. Activated macrophages are key drivers of vascular inflammation in KD. The aim of this study was to examine differences in M1 and M2 macrophage marker expression in patients with KD. Blood samples were obtained from 18 healthy controls and 18 patients with KD at 24 h prior and 21 days after to intravenous immunoglobulin therapy. GeneChip Human Transcriptome Array 2.0 and Illumina HumanMethylation450 BeadChip were used to examined the mRNA expression and corresponding CpG site methylation ratios of 10 M1 surface markers and 15 M2 surface markers. Of the markers examined 2 M1 markers (TLR2, IL2RA) and 8 M2 markers (ARG1, CCR2, TLR1, TLR8, TLR5, MS4A6A, CD36, and MS4A4A) showed increased mRNA expression in the acute phase of KD which decreased after IVIG therapy (P < 0.05). Corresponding CpG sites in the promoter regions these markers were hypomethylated in the acute phase of KD and significantly increased after IVIG therapy. In conclusion, both M1 and M2 markers showed increased mRNA expression in the acute phase of KD. CpG site methylation may be one of the mechanisms governing macrophage polarization in KD.

Original languageEnglish
Article number129
JournalFrontiers in Pediatrics
Volume8
DOIs
StatePublished - 03 04 2020

Bibliographical note

Publisher Copyright:
© Copyright © 2020 Guo, Chang, Huang, Wang and Kuo.

Keywords

  • Kawasaki disease
  • macrophage
  • methylation
  • methylation array
  • transcriptome array

Fingerprint

Dive into the research topics of 'Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease'. Together they form a unique fingerprint.

Cite this