Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice

Hui Chun Chu; Hsing Ying Lee; Yen Shu Huang; Wei Lien Tseng; Ching Ju Yen; Ju Chien Cheng; Ching Ping Tseng

doi:10.1016/j.febslet.2013.11.002

Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice

Hui Chun Chu, Hsing Ying Lee, Yen Shu Huang, Wei Lien Tseng, Ching Ju Yen, Ju Chien Cheng^*, Ching Ping Tseng

^*Corresponding author for this work

Department of Medical Biotechnology and Laboratory Science

Research output: Contribution to journal › Journal Article › peer-review

11 Scopus citations

Abstract

Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119⁺CD71⁺ erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined.

Original language	English
Pages (from-to)	58-64
Number of pages	7
Journal	FEBS Letters
Volume	588
Issue number	1
DOIs	https://doi.org/10.1016/j.febslet.2013.11.002
State	Published - 03 01 2014

Keywords

Erythroid differentiation
K562 cell
Reeler mice
Reelin
Sodium butyrate

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10.1016/j.febslet.2013.11.002

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title = "Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice",

abstract = "Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119+CD71+ erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined.",

keywords = "Erythroid differentiation, K562 cell, Reeler mice, Reelin, Sodium butyrate",

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T1 - Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice

AU - Chu, Hui Chun

AU - Lee, Hsing Ying

AU - Huang, Yen Shu

AU - Tseng, Wei Lien

AU - Yen, Ching Ju

AU - Cheng, Ju Chien

AU - Tseng, Ching Ping

PY - 2014/1/3

Y1 - 2014/1/3

N2 - Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119+CD71+ erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined.

AB - Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119+CD71+ erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined.

KW - Erythroid differentiation

KW - K562 cell

KW - Reeler mice

KW - Reelin

KW - Sodium butyrate

UR - https://www.scopus.com/pages/publications/84891019145

U2 - 10.1016/j.febslet.2013.11.002

DO - 10.1016/j.febslet.2013.11.002

M3 - 文章

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AN - SCOPUS:84891019145

SN - 0014-5793

VL - 588

SP - 58

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JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice

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Keywords

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