TY - JOUR
T1 - Erythropoietin markedly attenuates brain infarct size and improves neurological function in the rat
AU - Chun-Man, Yuen
AU - Steve, Leu
AU - Fan-Yen, Lee
AU - Chia-Hung, Yen
AU - Yu-Chun, Lin
AU - Sarah, Chua
AU - Sheng-Ying, Chung
AU - Han-Tan, Chai
AU - Jiunn-Jye, Sheu
AU - Sheung-Fat, Ko
AU - Cheuk-Kwan, Sun
AU - Hon-Kan, Yip
PY - 2010/10
Y1 - 2010/10
N2 - Background: The impact of epoetin beta (recombinant human erythropoietin) on brain infarction area (BIA) and neurological status in a rat model of acute ischemic stroke (IS) induced by distal left internal carotid artery occlusion was investigated. Methods: Adult male Sprague-Dawley rats (n = 30) were categorized into group 2 (IS only) and group 3 (IS plus intraperitoneal erythropoietin 5000 IU/kg at 0, 12, and 24 hours after IS). Healthy Sprague-Dawley rats (n = 10) served as group 1. Results: Analysis of brain tissues showed larger BIA in group 2 than in group 3 (P < 0.001). Corner test identified highest frequency of left turn in group 2 (P < 0.05). The mRNA expressions of Bax, caspase 3, interleukin 18, toll-like receptor 4, and plasminogen activator inhibitor 1 were highest, whereas Bcl-2 was lowest in group 2 (P < 0.05). Lower CXCR4 and stromal cell-derived factor 1 expressions were noted in group 2 than in group 3 (P < 0.01). Immunohistofluorescence staining showed lower expressions of CXCR4, stromal cell-derived factor 1, von Willebrand factor, and doublecortin with higher number of apoptotic nuclei in group 2 than in group 3 (P < 0.001). Immunohistochemical staining demonstrated lower cellular proliferation and number of small vessels with higher glial fibrillary acid protein expression in group 2 than in group 3 (P < 0.01). Conclusions: Erythropoietin significantly limited BIA and improved sensorimotor dysfunction after acute IS.
AB - Background: The impact of epoetin beta (recombinant human erythropoietin) on brain infarction area (BIA) and neurological status in a rat model of acute ischemic stroke (IS) induced by distal left internal carotid artery occlusion was investigated. Methods: Adult male Sprague-Dawley rats (n = 30) were categorized into group 2 (IS only) and group 3 (IS plus intraperitoneal erythropoietin 5000 IU/kg at 0, 12, and 24 hours after IS). Healthy Sprague-Dawley rats (n = 10) served as group 1. Results: Analysis of brain tissues showed larger BIA in group 2 than in group 3 (P < 0.001). Corner test identified highest frequency of left turn in group 2 (P < 0.05). The mRNA expressions of Bax, caspase 3, interleukin 18, toll-like receptor 4, and plasminogen activator inhibitor 1 were highest, whereas Bcl-2 was lowest in group 2 (P < 0.05). Lower CXCR4 and stromal cell-derived factor 1 expressions were noted in group 2 than in group 3 (P < 0.01). Immunohistofluorescence staining showed lower expressions of CXCR4, stromal cell-derived factor 1, von Willebrand factor, and doublecortin with higher number of apoptotic nuclei in group 2 than in group 3 (P < 0.001). Immunohistochemical staining demonstrated lower cellular proliferation and number of small vessels with higher glial fibrillary acid protein expression in group 2 than in group 3 (P < 0.01). Conclusions: Erythropoietin significantly limited BIA and improved sensorimotor dysfunction after acute IS.
KW - Acute ischemic stroke
KW - EPO treatment
UR - http://www.scopus.com/inward/record.url?scp=85008256418&partnerID=8YFLogxK
U2 - 10.2310/jim.0b013e3181e80c40
DO - 10.2310/jim.0b013e3181e80c40
M3 - 文章
C2 - 20601898
AN - SCOPUS:85008256418
SN - 1081-5589
VL - 58
SP - 893
EP - 904
JO - Journal of Investigative Medicine
JF - Journal of Investigative Medicine
IS - 7
ER -