Essential role of PKCδ in histone deacetylase inhibitor-induced Epstein-Barr virus reactivation in nasopharyngeal carcinoma cells

Heng Huan Lee; Shih Shin Chang; Sue Jane Lin; Huey Huey Chua; Tze Jiun Tsai; Kevin Tsai; You Chang Lo; Hong Chen Chen; Ching Hwa Tsai

doi:10.1099/vir.0.83533-0

Essential role of PKCδ in histone deacetylase inhibitor-induced Epstein-Barr virus reactivation in nasopharyngeal carcinoma cells

Heng Huan Lee
, Shih Shin Chang
, Sue Jane Lin
, Huey Huey Chua
, Tze Jiun Tsai
, Kevin Tsai
, You Chang Lo
, Hong Chen Chen
, Ching Hwa Tsai^*

^*Corresponding author for this work

National Taiwan University
University of Massachusetts Medical School
National Chung Hsing University

Research output: Contribution to journal › Journal Article › peer-review

18 Scopus citations

Abstract

Histone deactylase inhibitors (HDACi) are common chemotherapeutic agents that stimulate Epstein-Barr virus (EBV) reactivation; the detailed mechanism remains obscure. In this study, it is demonstrated that PKCδ is required for induction of the EBV lytic cycle by HDACi. Inhibition of PKCδ abrogates HDACi-mediated transcriptional activation of the Zta promoter and downstream lytic gene expression. Nuclear translocation of PKCδ is observed following HDACi stimulation and its overexpression leads to progression of the EBV lytic cycle. Our study suggests that PKCδ is a crucial mediator of EBV reactivation and provides a novel insight to study the regulation of the EBV lytic cycle.

Original language	English
Pages (from-to)	878-883
Number of pages	6
Journal	Journal of General Virology
Volume	89
Issue number	4
DOIs	https://doi.org/10.1099/vir.0.83533-0
State	Published - 04 2008
Externally published	Yes

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10.1099/vir.0.83533-0

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title = "Essential role of PKCδ in histone deacetylase inhibitor-induced Epstein-Barr virus reactivation in nasopharyngeal carcinoma cells",

abstract = "Histone deactylase inhibitors (HDACi) are common chemotherapeutic agents that stimulate Epstein-Barr virus (EBV) reactivation; the detailed mechanism remains obscure. In this study, it is demonstrated that PKCδ is required for induction of the EBV lytic cycle by HDACi. Inhibition of PKCδ abrogates HDACi-mediated transcriptional activation of the Zta promoter and downstream lytic gene expression. Nuclear translocation of PKCδ is observed following HDACi stimulation and its overexpression leads to progression of the EBV lytic cycle. Our study suggests that PKCδ is a crucial mediator of EBV reactivation and provides a novel insight to study the regulation of the EBV lytic cycle.",

author = "Lee, \{Heng Huan\} and Chang, \{Shih Shin\} and Lin, \{Sue Jane\} and Chua, \{Huey Huey\} and Tsai, \{Tze Jiun\} and Kevin Tsai and Lo, \{You Chang\} and Chen, \{Hong Chen\} and Tsai, \{Ching Hwa\}",

year = "2008",

month = apr,

doi = "10.1099/vir.0.83533-0",

language = "英语",

volume = "89",

pages = "878--883",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Microbiology Society",

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TY - JOUR

T1 - Essential role of PKCδ in histone deacetylase inhibitor-induced Epstein-Barr virus reactivation in nasopharyngeal carcinoma cells

AU - Lee, Heng Huan

AU - Chang, Shih Shin

AU - Lin, Sue Jane

AU - Chua, Huey Huey

AU - Tsai, Tze Jiun

AU - Tsai, Kevin

AU - Lo, You Chang

AU - Chen, Hong Chen

AU - Tsai, Ching Hwa

PY - 2008/4

Y1 - 2008/4

N2 - Histone deactylase inhibitors (HDACi) are common chemotherapeutic agents that stimulate Epstein-Barr virus (EBV) reactivation; the detailed mechanism remains obscure. In this study, it is demonstrated that PKCδ is required for induction of the EBV lytic cycle by HDACi. Inhibition of PKCδ abrogates HDACi-mediated transcriptional activation of the Zta promoter and downstream lytic gene expression. Nuclear translocation of PKCδ is observed following HDACi stimulation and its overexpression leads to progression of the EBV lytic cycle. Our study suggests that PKCδ is a crucial mediator of EBV reactivation and provides a novel insight to study the regulation of the EBV lytic cycle.

AB - Histone deactylase inhibitors (HDACi) are common chemotherapeutic agents that stimulate Epstein-Barr virus (EBV) reactivation; the detailed mechanism remains obscure. In this study, it is demonstrated that PKCδ is required for induction of the EBV lytic cycle by HDACi. Inhibition of PKCδ abrogates HDACi-mediated transcriptional activation of the Zta promoter and downstream lytic gene expression. Nuclear translocation of PKCδ is observed following HDACi stimulation and its overexpression leads to progression of the EBV lytic cycle. Our study suggests that PKCδ is a crucial mediator of EBV reactivation and provides a novel insight to study the regulation of the EBV lytic cycle.

UR - https://www.scopus.com/pages/publications/45549096221

U2 - 10.1099/vir.0.83533-0

DO - 10.1099/vir.0.83533-0

M3 - 文章

C2 - 18343827

AN - SCOPUS:45549096221

SN - 0022-1317

VL - 89

SP - 878

EP - 883

JO - Journal of General Virology

JF - Journal of General Virology

IS - 4

ER -

Essential role of PKCδ in histone deacetylase inhibitor-induced Epstein-Barr virus reactivation in nasopharyngeal carcinoma cells

Abstract

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