Establishment and Characterization of a Tumor Cell Line from Human Nasopharyngeal Carcinoma Tissue

Yu Sun Chang, Su Yu Lin, Pey Fen Lee, Tim Durff, Ho Chin Chung, Min Shiu Tsai

Research output: Contribution to journalJournal Article peer-review

26 Scopus citations

Abstract

An epithelial tumor cell line, CGI, was established from human nasopharyngeal carcinoma tissues. The CGI cells are of an epithelial origin as shown by their reactivities with the epithelial-specific anti-keratin antibodies and by the presence of the desmosome structure at cell-cell junctions. CGI cells possess characteristics of tumor cells because these cells are tumorigenic in nude mice and also have reduced serum requirements for in vitro cultivation. The doubling time of CGI cells is 20 h and these cells have been successfully cultured in vitro for more than 200 generations. The average chromosome number of these cells is 60. Slot and Southern blot hybridizations showed the presence of Epstein-Barr virus-DNA sequences in CGI cells. This cell line provides us an in vitro system for the study of the role of Epstein-Barr virus in nasopharyngeal carcinoma.

Original languageEnglish
Pages (from-to)6752-6757
Number of pages6
JournalCancer Research
Volume49
Issue number23
StatePublished - 01 12 1989
Externally publishedYes

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