Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats

Chun hung Yang, Heng yuan Chang, Yi chuan Chen, Chia chen Lu, Shyh shyun Huang, Guan jhong Huang, Hsin chih Lai*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

4 Scopus citations

Abstract

Objective: To study whether the ethanol extract of Phellinus merrillii (EPM) has chemopreventive potential against liver carcinogenesis. Methods: Thirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine (DEN)-initiated, 2-acetylaminofluorene (2-AAF) and partial hepatectomy (PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase (sGOT), glutamic pyruvic transaminase (sGPT) and gamma-glutamyl transpeptidase (γ-GT) were measured. Results: Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of sGOT, sGPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group (P<0.05 or P<0.01). These phenotypes were accompanied by a significant increase in natural killer cell activity. Conclusion: EPM showed a strong liver preventive effect against DEN+2-AAF+PH-induced hepatocarcinogenesis in a rat model.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalChinese Journal of Integrative Medicine
Volume23
Issue number2
DOIs
StatePublished - 01 02 2017

Bibliographical note

Publisher Copyright:
© 2016, Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg.

Keywords

  • 2-acetylaminofluorene
  • Phellinus merrillii
  • chemoprevention
  • diethylnitrosamine
  • hepatocarcinogenesis

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