Etomidate suppresses invasion and migration of human A549 Lung Adenocarcinoma Cells

Chin Nan Chu, King Chuen Wu, Wai Shan Chung, Li Cheng Zheng, Ta Kuo Juan, Yung Ting Hsiao, Shu Fen Peng, Jung Long Yang, Yi Shih Ma, Rick Sai Chuen Wu, Jing Gung Chung*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

19 Scopus citations

Abstract

Background/Aim: Etomidate, an intravenous anesthetic, has been shown to have anticancer effects, including induction of cell-cycle arrest and apoptosis. However, to our knowledge, there are no reports about the anti-metastasis effects of etomidate on A549 human lung adenocarcinoma cells. Materials and Methods: The cell viability, cell adhesion, gelatin zymography assay, transwell migration and invasion assay, and western blotting analysis were used to investigate the effects of etomidate on A549 cells. Results: In our study, etomidate showed low cytotoxicity, inhibited cell adhesion, and suppressed the migration and invasion in A549 cells. The activity of matrix metallopeptidase 2 (MMP2) was reduced by 48 h treatment of etomidate. Results of western blotting analysis indicated that etomidate downregulated the expression of protein kinase C, MMP7, MMP1, MMP9, and p-p-38, but up-regulated that of RAS, phosphoinositide 3-kinase, and phosphor-extracellular-signal related kinase after 24 and 48 h treatment, in A549 cells. Conclusion: Etomidate suppressed the migration and invasion of lung adenocarcinoma A549 cells via inhibiting the expression of MMP1, MMP2, MMP7 and MMP9, and provides potential therapeutic targets for lung cancer treatment.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalAnticancer Research
Volume39
Issue number1
DOIs
StatePublished - 01 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 International Institute of Anticancer Research. All Rights Reserved.

Keywords

  • Etomidate
  • MMPs
  • human lung adenocarcinoma epithelial A549 cells
  • invasion
  • migration

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