Etv5a regulates the proliferation of ventral mesoderm cells and the formation of hemato-vascular derivatives

Shin Yi Chen, Hung Yu Shih, Sheng Jia Lin, Chung Der Hsiao, Zih Cing Li, Yi Chuan Cheng*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

Hematopoietic and vascular endothelial cells constitute the circulatory system and are both generated from the ventral mesoderm. However, the molecules and signaling pathways involved in ventral mesoderm formation and specification remain unclear. We found that zebrafish etv5a was expressed in the ventral mesoderm during gastrulation. Knockdown of Etv5a using morpholinos increased the proliferation of ventral mesoderm cells and caused defects in hematopoietic derivatives and in vascular formation. By contrast, the formation of other mesodermal derivatives, such as pronephros, somites and the gut wall, was not affected. Knockdown specificity was further confirmed by overexpression of an etv5a construct lacking its acidic domain. In conclusion, our data reveal that etv5a is essential for the inhibition of ventral mesoderm cell proliferation and for the formation of the hemato-vascular lineage.

Original languageEnglish
Pages (from-to)5626-5634
Number of pages9
JournalJournal of Cell Science
Volume126
Issue number24
DOIs
StatePublished - 15 12 2013

Keywords

  • Etv5a
  • Hemato-vascular derivatives
  • Ventral mesoderm

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