@inproceedings{e698e57408e8434faada28e1ed9a6a19,
title = "EWOD based microfluidic system with coplanar electrodes for parallel DNA ligation of ultramicro volume",
abstract = "This paper reports the completion of a parallel ultramicro-volume DNA ligation processes using a coplanar electrode type of electrowetting-on- dielectric (EWOD) microfluidic system. The droplets, containing DNA, a ligation enzyme and a multi-salt reaction buffer, served to complete the ligation using a developed EWOD system. In order to reduce the effect from environment, so the concept of core-shell droplet was used in this study. Droplets of ultramicro volume (0.3μL) were successfully generated from reservoirs between one plate with coplanar electrodes and another plate with a hydrophobic surface free of electrodes. In one successful cloning, the total usage of reagents in an ultramicro-volume EWOD chip was 2.1μL; no volume was wasted, in comparison with 85% waste with the standard protocol (15μL). The results also show that the four parallel DNA ligation processes were accomplished without damage to the chip surface and without biomaterial annulment.",
keywords = "Coplanar, DNA ligation, EWOD, Ultramicro volume",
author = "Lin, {H. C.} and Liu, {Y. J.} and Yao, {D. J.} and Chang, {H. Y.}",
year = "2009",
doi = "10.1109/SENSOR.2009.5285557",
language = "英语",
isbn = "9781424441938",
series = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
pages = "92--95",
booktitle = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems",
note = "TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems ; Conference date: 21-06-2009 Through 25-06-2009",
}