Exogenous BMP7 administration attenuated vascular calcification and improved bone disorders in chronic uremic rats

Chien Te Lee*, Wei Hung Kuo, You Lin Tain, Yi Wang, Wen Chin Lee

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations

Abstract

Vascular calcification is commonly observed in chronic kidney disease (CKD) and is associated with increased morbidity and mortality. This study examined whether exogenous BMP7 administration can modulate disturbed CKD-MBD in adenine-induced chronic uremic rats. After an adenine diet for 4 weeks, the animals were injected with BMP7 for 2 weeks. Biochemical data, kidney tissue, bony structure, and vascular calcification of the thoracic aorta were examined and compared. Reduced renal function, hyperphosphatemia, and hyperparathyroidism with low 1,25(OH)2 vitamin D levels were observed in the adenine group. MicroCT revealed reduced bone mineral density (BMD), decreased bone and tissue volume ratio (BV/TV), and decreased trabecular number with increased separation. Marked vascular calcification was observed in adenine-fed animals, and immunohistochemical analysis showed increased expression of BMP2, RUNX2, vitamin D receptor (VDR), and Pit1 in aortic tissue. Treatment with BMP7 was associated with reduced serum phosphate, intact parathyroid hormone, FGF23, sclerostin, and DKK1 levels. BMP7 administration was accompanied with improvements in BMD and BV/TV. The increase in BMP2, RUNX2, VDR, and Pit1 was reversed by BMP7. In conclusion, exogenous BMP7 administration improved hyperphosphatemia and hyperparathyroidism in adenine-induced CKD. This treatment also attenuated vascular calcification and modulated structural abnormalities in the skeletal system.

Original languageEnglish
Pages (from-to)8-13
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume621
DOIs
StatePublished - 17 09 2022

Bibliographical note

Publisher Copyright:
© 2022 The Authors

Keywords

  • BMP7
  • Chronic kidney disease
  • Mineral and bone disorder
  • Vascular calcification

Fingerprint

Dive into the research topics of 'Exogenous BMP7 administration attenuated vascular calcification and improved bone disorders in chronic uremic rats'. Together they form a unique fingerprint.

Cite this