Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells

Yu Cheng Chen; Wei Cheng Tsai; Zhi Xiang Li; Wan Jung Lin; Hao Yu Lin; Yi Ju Hsieh; Kai Hsuan Wang; You Yan Chen; Tsong Long Hwang; Tzou Yien Lin

doi:10.1371/journal.pone.0320154

Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells

Yu Cheng Chen
, Wei Cheng Tsai
, Zhi Xiang Li
, Wan Jung Lin
, Hao Yu Lin
, Yi Ju Hsieh
, Kai Hsuan Wang
, You Yan Chen
, Tsong Long Hwang^*
, Tzou Yien Lin^*

^*Corresponding author for this work

Chang Gung University of Science and Technology
Ltd.
Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

10 Scopus citations

Abstract

Human hair dermal papilla cells (HHDPCs) play a significant role in hair growth. This study found that human umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Es) effectively enhanced cell growth of HHDPCs. UC-MSC-Es has a size range of 30–180 nm and expression of CD9, CD63, CD81, CD73, and TSG101. UC-MSC-Es significantly increased cell populations of HHDPCs in the S and G2/M phases. UC-MSC-Es also increased the expression of cell cycle-related proteins, β-catenin, and cyclin D1. Further mechanistic studies demonstrated that UC-MSC-Es promoted the phosphorylation of Akt and GSK-3β, and the inhibition of PI3K and Akt reduced the proliferative effects of UC-MSC-Es. Collectively, these findings suggest that UC-MSC-Es have a potential effect in treating hair loss through modulating PI3K and Akt-dependent pathways in HHDPCs.

Original language	English
Article number	e0320154
Pages (from-to)	e0320154
Journal	PLoS ONE
Volume	20
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0320154
State	Published - 04 2025

Bibliographical note

Copyright: © 2025 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Keywords

Humans
Exosomes/metabolism
Mesenchymal Stem Cells/cytology
Umbilical Cord/cytology
Proto-Oncogene Proteins c-akt/metabolism
Hair Follicle/cytology
Cell Proliferation
Phosphatidylinositol 3-Kinases/metabolism
beta Catenin/metabolism
Cells, Cultured
Glycogen Synthase Kinase 3 beta
Signal Transduction
Dermis/cytology
Glycogen Synthase Kinase 3/metabolism
Phosphorylation
Cell Cycle
Cyclin D1/metabolism
Hair/growth & development

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10.1371/journal.pone.0320154

Cite this

@article{43d1289098d849f8aaf8263bbebfdb84,

title = "Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells",

abstract = "Human hair dermal papilla cells (HHDPCs) play a significant role in hair growth. This study found that human umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Es) effectively enhanced cell growth of HHDPCs. UC-MSC-Es has a size range of 30{\textendash}180 nm and expression of CD9, CD63, CD81, CD73, and TSG101. UC-MSC-Es significantly increased cell populations of HHDPCs in the S and G2/M phases. UC-MSC-Es also increased the expression of cell cycle-related proteins, β-catenin, and cyclin D1. Further mechanistic studies demonstrated that UC-MSC-Es promoted the phosphorylation of Akt and GSK-3β, and the inhibition of PI3K and Akt reduced the proliferative effects of UC-MSC-Es. Collectively, these findings suggest that UC-MSC-Es have a potential effect in treating hair loss through modulating PI3K and Akt-dependent pathways in HHDPCs.",

keywords = "Humans, Exosomes/metabolism, Mesenchymal Stem Cells/cytology, Umbilical Cord/cytology, Proto-Oncogene Proteins c-akt/metabolism, Hair Follicle/cytology, Cell Proliferation, Phosphatidylinositol 3-Kinases/metabolism, beta Catenin/metabolism, Cells, Cultured, Glycogen Synthase Kinase 3 beta, Signal Transduction, Dermis/cytology, Glycogen Synthase Kinase 3/metabolism, Phosphorylation, Cell Cycle, Cyclin D1/metabolism, Hair/growth \& development",

author = "Chen, \{Yu Cheng\} and Tsai, \{Wei Cheng\} and Li, \{Zhi Xiang\} and Lin, \{Wan Jung\} and Lin, \{Hao Yu\} and Hsieh, \{Yi Ju\} and Wang, \{Kai Hsuan\} and Chen, \{You Yan\} and Hwang, \{Tsong Long\} and Lin, \{Tzou Yien\}",

note = "Copyright: {\textcopyright} 2025 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2025",

month = apr,

doi = "10.1371/journal.pone.0320154",

language = "英语",

volume = "20",

pages = "e0320154",

journal = "PLoS ONE",

issn = "1932-6203",

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TY - JOUR

T1 - Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells

AU - Chen, Yu Cheng

AU - Tsai, Wei Cheng

AU - Li, Zhi Xiang

AU - Lin, Wan Jung

AU - Lin, Hao Yu

AU - Hsieh, Yi Ju

AU - Wang, Kai Hsuan

AU - Chen, You Yan

AU - Hwang, Tsong Long

AU - Lin, Tzou Yien

N1 - Copyright: © 2025 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2025/4

Y1 - 2025/4

N2 - Human hair dermal papilla cells (HHDPCs) play a significant role in hair growth. This study found that human umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Es) effectively enhanced cell growth of HHDPCs. UC-MSC-Es has a size range of 30–180 nm and expression of CD9, CD63, CD81, CD73, and TSG101. UC-MSC-Es significantly increased cell populations of HHDPCs in the S and G2/M phases. UC-MSC-Es also increased the expression of cell cycle-related proteins, β-catenin, and cyclin D1. Further mechanistic studies demonstrated that UC-MSC-Es promoted the phosphorylation of Akt and GSK-3β, and the inhibition of PI3K and Akt reduced the proliferative effects of UC-MSC-Es. Collectively, these findings suggest that UC-MSC-Es have a potential effect in treating hair loss through modulating PI3K and Akt-dependent pathways in HHDPCs.

AB - Human hair dermal papilla cells (HHDPCs) play a significant role in hair growth. This study found that human umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Es) effectively enhanced cell growth of HHDPCs. UC-MSC-Es has a size range of 30–180 nm and expression of CD9, CD63, CD81, CD73, and TSG101. UC-MSC-Es significantly increased cell populations of HHDPCs in the S and G2/M phases. UC-MSC-Es also increased the expression of cell cycle-related proteins, β-catenin, and cyclin D1. Further mechanistic studies demonstrated that UC-MSC-Es promoted the phosphorylation of Akt and GSK-3β, and the inhibition of PI3K and Akt reduced the proliferative effects of UC-MSC-Es. Collectively, these findings suggest that UC-MSC-Es have a potential effect in treating hair loss through modulating PI3K and Akt-dependent pathways in HHDPCs.

KW - Humans

KW - Exosomes/metabolism

KW - Mesenchymal Stem Cells/cytology

KW - Umbilical Cord/cytology

KW - Proto-Oncogene Proteins c-akt/metabolism

KW - Hair Follicle/cytology

KW - Cell Proliferation

KW - Phosphatidylinositol 3-Kinases/metabolism

KW - beta Catenin/metabolism

KW - Cells, Cultured

KW - Glycogen Synthase Kinase 3 beta

KW - Signal Transduction

KW - Dermis/cytology

KW - Glycogen Synthase Kinase 3/metabolism

KW - Phosphorylation

KW - Cell Cycle

KW - Cyclin D1/metabolism

KW - Hair/growth & development

UR - https://www.scopus.com/pages/publications/105004219153

U2 - 10.1371/journal.pone.0320154

DO - 10.1371/journal.pone.0320154

M3 - 文章

C2 - 40305498

AN - SCOPUS:105004219153

SN - 1932-6203

VL - 20

SP - e0320154

JO - PLoS ONE

JF - PLoS ONE

IS - 4

M1 - e0320154

ER -

Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells

Abstract

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Keywords

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