Abstract
Mesenchymal stem cells (MSCs) isolated from Wharton’s jelly (WJ-MSCs) and adipose tissue (AD-MSCs) are alternative sources for bone marrow-derived MSCs. Owing to their multiple functions in angiogenesis, immune modulation, proliferation, migration, and nerve regeneration, MSC-derived exosomes can be applied in “cell-free cell therapy”. Here, we investigated the functional protein components between the exosomes from WJ-MSCs and AD-MSCs to explain their distinct functions. Proteins of WJ-MSC and AD-MSC exosomes were collected and compared based on iTRAQ gel-free proteomics data. Results: In total, 1695 proteins were detected in exosomes. Of these, 315 were more abundant (>1.25-fold) in AD-MSC exosomes and 362 kept higher levels in WJ-MSC exosomes, including fibrinogen proteins. Pathway enrichment analysis suggested that WJ-MSC exosomes had higher potential for wound healing than AD-MSC exosomes. Therefore, we treated keratinocyte cells with exosomes and the recombinant protein of fibrinogen beta chain (FGB). It turned out that WJ-MSC exosomes better promoted keratinocyte growth and migration than AD-MSC exosomes. In addition, FGB treatment had similar results to WJ-MSC exosomes. The fact that WJ-MSC exosomes promoted keratinocyte growth and migration better than AD-MSC exosomes can be explained by their higher FGB abundance. Exploring the various components of AD-MSC and WJ-MSC exosomes can aid in their different clinical applications.
Original language | English |
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Article number | 4758 |
Journal | International Journal of Molecular Sciences |
Volume | 25 |
Issue number | 9 |
DOIs | |
State | Published - 26 04 2024 |
Bibliographical note
Publisher Copyright:© 2024 by the authors.
Keywords
- adipose tissue mesenchymal stem cell
- exosome
- fibrinogen beta chain
- iTRAQ
- Wharton’s jelly mesenchymal stem cell
- Cell Proliferation
- Humans
- Cells, Cultured
- Exosomes/metabolism
- Keratinocytes/metabolism
- Mesenchymal Stem Cells/metabolism
- Wharton Jelly/cytology
- Wound Healing
- Fibrinogen/metabolism
- Proteome/metabolism
- Proteomics/methods
- Adipose Tissue/cytology
- Cell Movement