Exosomes Secreted by Wharton’s Jelly-Derived Mesenchymal Stem Cells Promote the Ability of Cell Proliferation and Migration for Keratinocyte

Hong Ren Yu, Hsin-Chun Huang, I. Lun Chen, Sung Chou Li*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

1 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) isolated from Wharton’s jelly (WJ-MSCs) and adipose tissue (AD-MSCs) are alternative sources for bone marrow-derived MSCs. Owing to their multiple functions in angiogenesis, immune modulation, proliferation, migration, and nerve regeneration, MSC-derived exosomes can be applied in “cell-free cell therapy”. Here, we investigated the functional protein components between the exosomes from WJ-MSCs and AD-MSCs to explain their distinct functions. Proteins of WJ-MSC and AD-MSC exosomes were collected and compared based on iTRAQ gel-free proteomics data. Results: In total, 1695 proteins were detected in exosomes. Of these, 315 were more abundant (>1.25-fold) in AD-MSC exosomes and 362 kept higher levels in WJ-MSC exosomes, including fibrinogen proteins. Pathway enrichment analysis suggested that WJ-MSC exosomes had higher potential for wound healing than AD-MSC exosomes. Therefore, we treated keratinocyte cells with exosomes and the recombinant protein of fibrinogen beta chain (FGB). It turned out that WJ-MSC exosomes better promoted keratinocyte growth and migration than AD-MSC exosomes. In addition, FGB treatment had similar results to WJ-MSC exosomes. The fact that WJ-MSC exosomes promoted keratinocyte growth and migration better than AD-MSC exosomes can be explained by their higher FGB abundance. Exploring the various components of AD-MSC and WJ-MSC exosomes can aid in their different clinical applications.

Original languageEnglish
Article number4758
JournalInternational Journal of Molecular Sciences
Volume25
Issue number9
DOIs
StatePublished - 26 04 2024

Bibliographical note

Publisher Copyright:
© 2024 by the authors.

Keywords

  • adipose tissue mesenchymal stem cell
  • exosome
  • fibrinogen beta chain
  • iTRAQ
  • Wharton’s jelly mesenchymal stem cell
  • Cell Proliferation
  • Humans
  • Cells, Cultured
  • Exosomes/metabolism
  • Keratinocytes/metabolism
  • Mesenchymal Stem Cells/metabolism
  • Wharton Jelly/cytology
  • Wound Healing
  • Fibrinogen/metabolism
  • Proteome/metabolism
  • Proteomics/methods
  • Adipose Tissue/cytology
  • Cell Movement

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