TY - JOUR
T1 - Exploring the recognized bio-mimicry materials for gas sensing
AU - Wu, Tzong Zeng
AU - Lo, Yen Ren
AU - Chan, Err Cheng
PY - 2001
Y1 - 2001
N2 - This study was undertaken to synthesize peptides that are partially similar to the binding sites of human olfactory receptor protein. First, a putative 3-D model structure of human olfactory receptor protein (P30953) was modeled using a molecular simulation method. The computer docking simulation was then performed to determine the most plausible binding sites between the model structure and target gases, trimethylamine, ammonia, acetic acid, and o-xylene. According to the simulation result, a series of polypeptide sequences, horp61 for TMA, horp103 for o-xylene, horp109 for ammonia, and horp193 for acetic acid as recognized molecules were designed for gas sensing purposes. Preparing these peptides as corresponding gas sensing probes, the results showed a high relative sensitivity response of 6.7 for TMA (probe horp61), 5.1 for o-xylene (probe horp103), 11 for ammonia (probe horp109), and 28 for acetic acid (probe horp193), respectively. These results indicate that peptide mimicking of binding domain on olfactory receptor opens a new window and offers a novel strategy for the further development of recognized materials for gas sensing.
AB - This study was undertaken to synthesize peptides that are partially similar to the binding sites of human olfactory receptor protein. First, a putative 3-D model structure of human olfactory receptor protein (P30953) was modeled using a molecular simulation method. The computer docking simulation was then performed to determine the most plausible binding sites between the model structure and target gases, trimethylamine, ammonia, acetic acid, and o-xylene. According to the simulation result, a series of polypeptide sequences, horp61 for TMA, horp103 for o-xylene, horp109 for ammonia, and horp193 for acetic acid as recognized molecules were designed for gas sensing purposes. Preparing these peptides as corresponding gas sensing probes, the results showed a high relative sensitivity response of 6.7 for TMA (probe horp61), 5.1 for o-xylene (probe horp103), 11 for ammonia (probe horp109), and 28 for acetic acid (probe horp193), respectively. These results indicate that peptide mimicking of binding domain on olfactory receptor opens a new window and offers a novel strategy for the further development of recognized materials for gas sensing.
KW - Docking
KW - Gas sensing probe
KW - Molecular recognition
KW - Olfactory receptor
KW - Synthetic peptide
UR - http://www.scopus.com/inward/record.url?scp=0034772024&partnerID=8YFLogxK
U2 - 10.1016/S0956-5663(01)00215-9
DO - 10.1016/S0956-5663(01)00215-9
M3 - 文章
C2 - 11679274
AN - SCOPUS:0034772024
SN - 0956-5663
VL - 16
SP - 945
EP - 953
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 9-12
ER -