Exposure to tris(2-butoxyethyl) phosphate induces abnormal sperm morphology and testicular histopathology in male rats

Hsiu Yung Pan; Fu Jen Cheng; Kuo Chen Huang; Chia Te Kung; Wan Ting Huang; Huey Ling You; Shau Hsuan Li; Chin Chou Wang; Wen Chin Lee; Ping Chi Hsu

doi:10.1016/j.ecoenv.2022.113718

Exposure to tris(2-butoxyethyl) phosphate induces abnormal sperm morphology and testicular histopathology in male rats

Hsiu Yung Pan, Fu Jen Cheng, Kuo Chen Huang, Chia Te Kung, Wan Ting Huang, Huey Ling You, Shau Hsuan Li, Chin Chou Wang, Wen Chin Lee, Ping Chi Hsu^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

8 Scopus citations

Abstract

Tris(2-butoxyethyl) phosphate (TBEP) is one of the most abundant organophosphate flame retardants in the environment. This study aimed to evaluate the effect of TBEP exposure during adolescence on male reproductive function in adult rats. Male Sprague–Dawley rats were treated with 20 and 200 mg/kg body weight of TBEP or corn oil from postnatal day (PND) 42 to PND 105. A significant increase in the proportion of sperm with abnormal morphology (flattened head and bent tail) and superoxide anion (O₂^-.) production in the sperm of the 200 mg/kg treated group was observed (p < 0.05). Excessive production of sperm hydrogen peroxide (H₂O₂) was found in both the 20 and 200 mg/kg treatment groups (p < 0.05). Disruption of testicular structure was observed in the 20 and 200 mg/kg treated groups and seminiferous tubule degeneration was observed in the 200 mg/kg treated group. Our study demonstrated the adverse effects of TBEP on male reproductive function in rats.

Original language	English
Article number	113718
Journal	Ecotoxicology and Environmental Safety
Volume	241
DOIs	https://doi.org/10.1016/j.ecoenv.2022.113718
State	Published - 08 2022

Bibliographical note

Publisher Copyright:
© 2022 The Authors

Keywords

Male reproductive function
Sperm morphology
TBEP
Tris(2-butoxyethyl) phosphate

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ecoenv.2022.113718

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title = "Exposure to tris(2-butoxyethyl) phosphate induces abnormal sperm morphology and testicular histopathology in male rats",

abstract = "Tris(2-butoxyethyl) phosphate (TBEP) is one of the most abundant organophosphate flame retardants in the environment. This study aimed to evaluate the effect of TBEP exposure during adolescence on male reproductive function in adult rats. Male Sprague–Dawley rats were treated with 20 and 200 mg/kg body weight of TBEP or corn oil from postnatal day (PND) 42 to PND 105. A significant increase in the proportion of sperm with abnormal morphology (flattened head and bent tail) and superoxide anion (O2-.) production in the sperm of the 200 mg/kg treated group was observed (p < 0.05). Excessive production of sperm hydrogen peroxide (H2O2) was found in both the 20 and 200 mg/kg treatment groups (p < 0.05). Disruption of testicular structure was observed in the 20 and 200 mg/kg treated groups and seminiferous tubule degeneration was observed in the 200 mg/kg treated group. Our study demonstrated the adverse effects of TBEP on male reproductive function in rats.",

keywords = "Male reproductive function, Sperm morphology, TBEP, Tris(2-butoxyethyl) phosphate",

author = "Pan, {Hsiu Yung} and Cheng, {Fu Jen} and Huang, {Kuo Chen} and Kung, {Chia Te} and Huang, {Wan Ting} and You, {Huey Ling} and Li, {Shau Hsuan} and Wang, {Chin Chou} and Lee, {Wen Chin} and Hsu, {Ping Chi}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = aug,

doi = "10.1016/j.ecoenv.2022.113718",

language = "英语",

volume = "241",

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AU - Pan, Hsiu Yung

AU - Cheng, Fu Jen

AU - Huang, Kuo Chen

AU - Kung, Chia Te

AU - Huang, Wan Ting

AU - You, Huey Ling

AU - Li, Shau Hsuan

AU - Wang, Chin Chou

AU - Lee, Wen Chin

AU - Hsu, Ping Chi

PY - 2022/8

Y1 - 2022/8

N2 - Tris(2-butoxyethyl) phosphate (TBEP) is one of the most abundant organophosphate flame retardants in the environment. This study aimed to evaluate the effect of TBEP exposure during adolescence on male reproductive function in adult rats. Male Sprague–Dawley rats were treated with 20 and 200 mg/kg body weight of TBEP or corn oil from postnatal day (PND) 42 to PND 105. A significant increase in the proportion of sperm with abnormal morphology (flattened head and bent tail) and superoxide anion (O2-.) production in the sperm of the 200 mg/kg treated group was observed (p < 0.05). Excessive production of sperm hydrogen peroxide (H2O2) was found in both the 20 and 200 mg/kg treatment groups (p < 0.05). Disruption of testicular structure was observed in the 20 and 200 mg/kg treated groups and seminiferous tubule degeneration was observed in the 200 mg/kg treated group. Our study demonstrated the adverse effects of TBEP on male reproductive function in rats.

AB - Tris(2-butoxyethyl) phosphate (TBEP) is one of the most abundant organophosphate flame retardants in the environment. This study aimed to evaluate the effect of TBEP exposure during adolescence on male reproductive function in adult rats. Male Sprague–Dawley rats were treated with 20 and 200 mg/kg body weight of TBEP or corn oil from postnatal day (PND) 42 to PND 105. A significant increase in the proportion of sperm with abnormal morphology (flattened head and bent tail) and superoxide anion (O2-.) production in the sperm of the 200 mg/kg treated group was observed (p < 0.05). Excessive production of sperm hydrogen peroxide (H2O2) was found in both the 20 and 200 mg/kg treatment groups (p < 0.05). Disruption of testicular structure was observed in the 20 and 200 mg/kg treated groups and seminiferous tubule degeneration was observed in the 200 mg/kg treated group. Our study demonstrated the adverse effects of TBEP on male reproductive function in rats.

KW - Male reproductive function

KW - Sperm morphology

KW - TBEP

KW - Tris(2-butoxyethyl) phosphate

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DO - 10.1016/j.ecoenv.2022.113718

M3 - 文章

C2 - 35660377

AN - SCOPUS:85131412464

SN - 0147-6513

VL - 241

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

M1 - 113718

ER -

Exposure to tris(2-butoxyethyl) phosphate induces abnormal sperm morphology and testicular histopathology in male rats

Abstract

Bibliographical note

Keywords

UN SDGs

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