TY - JOUR
T1 - Expression of estrogen receptors alfa and beta mRNA and alkaline phosphatase in the differentiation of osteoblasts from elderly postmenopausal women
T2 - Comparison with osteoblasts from osteosarcoma cell lines
AU - Chen, Fang Ping
AU - Hsu, Todd
AU - Hu, Chin Hwa
AU - Wang, Wen Der
AU - Wang, Kun Chuang
AU - Teng, Li Fen
PY - 2006/12
Y1 - 2006/12
N2 - Objective: To evaluate the expression of estrogen receptors (ER) α and β, and activity of alkaline phosphatase during differentiation of primary osteoblast cells (hOB) from aged postmenopausal women and human osteosarcoma cell lines (HOS, MG63). Materials and Methods: Osteoblast cultures were prepared from the upper femur of postmenopausal patients (age, 60-74 years) and HOS. At the indicated times (days 5, 10, 15, 20, and 25), alkaline phosphatase activity and expression of ERα and ERβ mRNA were evaluated. Results: In both cultures of primary hOB and HOS, alkaline phosphatase activity decreased at the osteoblast proliferation stage, whereas it subsequently increased at the matrix maturation stage. ERβ mRNA was strongly expressed in HOS on day 15 and remained at high levels of transcription through to day 25 (matrix maturation phase), whereas ERα mRNA was barely detectable during osteoblast differentiation. In hOB, transcription of ERα mRNA was much stronger than that of ERβ mRNA. Conclusion: The presence of ERα and ERβ mRNA in osteoblasts supports the involvement of estrogen in human bone formation. The developmental expression of alkaline phosphatase was not correlated to ER mRNA expression during osteoblast differentiation. ER isoforms may have different functions or interact with each other during osteoblast differentiation. Since the expression of ER isoforms is different between postmenopausal women and osteosarcoma cell lines, characteristics of osteosarcoma cell lines may not be suitable as a model for the evaluation of estrogen effects on postmenopausal osteoporosis.
AB - Objective: To evaluate the expression of estrogen receptors (ER) α and β, and activity of alkaline phosphatase during differentiation of primary osteoblast cells (hOB) from aged postmenopausal women and human osteosarcoma cell lines (HOS, MG63). Materials and Methods: Osteoblast cultures were prepared from the upper femur of postmenopausal patients (age, 60-74 years) and HOS. At the indicated times (days 5, 10, 15, 20, and 25), alkaline phosphatase activity and expression of ERα and ERβ mRNA were evaluated. Results: In both cultures of primary hOB and HOS, alkaline phosphatase activity decreased at the osteoblast proliferation stage, whereas it subsequently increased at the matrix maturation stage. ERβ mRNA was strongly expressed in HOS on day 15 and remained at high levels of transcription through to day 25 (matrix maturation phase), whereas ERα mRNA was barely detectable during osteoblast differentiation. In hOB, transcription of ERα mRNA was much stronger than that of ERβ mRNA. Conclusion: The presence of ERα and ERβ mRNA in osteoblasts supports the involvement of estrogen in human bone formation. The developmental expression of alkaline phosphatase was not correlated to ER mRNA expression during osteoblast differentiation. ER isoforms may have different functions or interact with each other during osteoblast differentiation. Since the expression of ER isoforms is different between postmenopausal women and osteosarcoma cell lines, characteristics of osteosarcoma cell lines may not be suitable as a model for the evaluation of estrogen effects on postmenopausal osteoporosis.
KW - Alkaline phosphatase
KW - Estrogen receptor α mRNA
KW - Estrogen receptor β mRNA
KW - Human osteosarcoma cell lines
KW - Postmenopausal women
KW - Primary human osteoblasts
UR - http://www.scopus.com/inward/record.url?scp=33846321175&partnerID=8YFLogxK
U2 - 10.1016/S1028-4559(09)60248-5
DO - 10.1016/S1028-4559(09)60248-5
M3 - 文章
C2 - 17175487
AN - SCOPUS:33846321175
SN - 1028-4559
VL - 45
SP - 307
EP - 312
JO - Taiwanese Journal of Obstetrics and Gynecology
JF - Taiwanese Journal of Obstetrics and Gynecology
IS - 4
ER -