TY - JOUR
T1 - Expression of Inflammatory and Neurogenic Mediators in Adenomyosis
AU - Carrarelli, Patrizia
AU - Yen, Chih Feng
AU - Funghi, Lucia
AU - Arcuri, Felice
AU - Tosti, Claudia
AU - Bifulco, Giuseppe
AU - Luddi, Alice
AU - Lee, Chyi Long
AU - Petraglia, Felice
N1 - Publisher Copyright:
© 2017 Society for Gynecologic Investigation.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Objective: Adenomyosis is a uterine disorder characterized by dysmenorrhea, dyspareunia, abnormal uterine bleeding, and infertility. Pathogenesis indicates that endometrial cells invade and proliferate within myometrium, and inflammatory mediators participate to the intense painful symptoms. The aim of the present study was to investigate the messenger RNA (mRNA) and protein expression of inflammatory (interleukin 1β [IL-1β], corticotropin-releasing hormone [CRH], urocortin [Ucn]) and neurogenic (nerve growth factors [NGFs], synaptophysin [SYN], microtubule-associated protein 2 [MAP2]) factors in adenomyotic nodules. Materials and Methods: This prospective study enrolled 16 women, 8 women with nodular adenomyosis and 8 control women undergoing to hysterectomy. Specimens from adenomyotic nodules and eutopic endometrium were collected after surgery. Endometrial tissue was also obtained from the control group and also used for preparing primary culture of human endometrial stromal cells (HESCs). Messenger RNA expression of inflammatory mediators (IL-1β, CRH, and Ucn) and neurogenic factors (NGF, SYN, and MAP2) was analyzed by real-time polymerase chain reaction. The in vitro effects of CRH/Ucn on NGF or SYN mRNA expression were also investigated. Results: Adenomyotic nodules highly expressed IL-1β, CRH, and Ucn mRNAs, as well as NGF, SYN, and MAP2 mRNAs (P <.001 vs eutopic endometrium and control). Endometrium of women with adenomyosis showed high expression of IL-1β and CRH (P <.001 vs control). Protein expression of CRH, NGF, and SYN in adenomyotic nodules was confirmed by immunohistochemical and immunofluorescence analyses. Urocortin increased NGF mRNA expression in cultured HESCs. Conclusion: The present study showed that adenomyotic nodules are novel site of expression of inflammatory and neurogenic factors, probably involved in the pathogenesis of adenomyosis.
AB - Objective: Adenomyosis is a uterine disorder characterized by dysmenorrhea, dyspareunia, abnormal uterine bleeding, and infertility. Pathogenesis indicates that endometrial cells invade and proliferate within myometrium, and inflammatory mediators participate to the intense painful symptoms. The aim of the present study was to investigate the messenger RNA (mRNA) and protein expression of inflammatory (interleukin 1β [IL-1β], corticotropin-releasing hormone [CRH], urocortin [Ucn]) and neurogenic (nerve growth factors [NGFs], synaptophysin [SYN], microtubule-associated protein 2 [MAP2]) factors in adenomyotic nodules. Materials and Methods: This prospective study enrolled 16 women, 8 women with nodular adenomyosis and 8 control women undergoing to hysterectomy. Specimens from adenomyotic nodules and eutopic endometrium were collected after surgery. Endometrial tissue was also obtained from the control group and also used for preparing primary culture of human endometrial stromal cells (HESCs). Messenger RNA expression of inflammatory mediators (IL-1β, CRH, and Ucn) and neurogenic factors (NGF, SYN, and MAP2) was analyzed by real-time polymerase chain reaction. The in vitro effects of CRH/Ucn on NGF or SYN mRNA expression were also investigated. Results: Adenomyotic nodules highly expressed IL-1β, CRH, and Ucn mRNAs, as well as NGF, SYN, and MAP2 mRNAs (P <.001 vs eutopic endometrium and control). Endometrium of women with adenomyosis showed high expression of IL-1β and CRH (P <.001 vs control). Protein expression of CRH, NGF, and SYN in adenomyotic nodules was confirmed by immunohistochemical and immunofluorescence analyses. Urocortin increased NGF mRNA expression in cultured HESCs. Conclusion: The present study showed that adenomyotic nodules are novel site of expression of inflammatory and neurogenic factors, probably involved in the pathogenesis of adenomyosis.
KW - adenomyosis
KW - inflammation
KW - neurogenesis
UR - https://www.scopus.com/pages/publications/85013001560
U2 - 10.1177/1933719116657192
DO - 10.1177/1933719116657192
M3 - 文章
C2 - 27440813
AN - SCOPUS:85013001560
SN - 1933-7191
VL - 24
SP - 369
EP - 375
JO - Reproductive Sciences
JF - Reproductive Sciences
IS - 3
ER -